食品添加劑中的L-色胺酸(L-Tryptophan, L-Trp)是人體中不可缺少的胺基酸。它對人體具有抗憂鬱及改善睡眠的功效，但當L-Trp過量的添加與在人體長期累積後，也容易在大腦中囤積導致幻覺，甚至引發精神分裂症。因此開發出一種能快速對L-色胺酸進行微量檢測的技術有其必要性。本研究先以溶膠凝膠法合成兩種粒徑，分別為234 nm及542 nm的二氧化矽奈米微球，再將二氧化矽奈米微球排列成單層陣列。並結合光子晶體和分子印記技術，獲得具有L-色胺酸辨識功能的反蛋白石孔洞狀結構，開發出一種新型的比色化學感測器，可快速又方便的進行檢測目標分子L-Trp。該感測器具有高靈敏度及特異性，由於L-Trp與甲基丙烯酸之間的氫鍵相互作用及特定的L-Trp分子識別過程導致水凝膠明顯溶脹，並以色度圖建立顏色與L-色胺酸的濃度變化關係，製作出可快速辨識L-色胺酸濃度的感測器。此外，本研究亦探討了水凝膠顆粒的大小及反蛋白石水凝膠的結構穩定性來比對L-Trp傳感器的靈敏度。

L-Tryptophan (L-Trp) in food additives is an amino acid that is not ingestible in the human body. It has anti-depressive and sleep-improving effects on the human body, but when L-Trp is excessively added and accumulated in the human body for a long time, it is also easy to accumulate in the brain to cause hallucinations and even trigger schizophrenia. Therefore, it is necessary to develop a technology that can quickly detect L-tryptophan in trace amounts. In this study, two forms of sol-gel method were synthesized, 234 nm and 542 nm silica nanospheres, and then the silica nanospheres were separated into a single-layer array. Combined with photonic crystal and molecular imprinting technology, a reverse-opal pore structure with L-tryptophan recognition function is obtained, and a new colorimetric chemical sensor is developed, which can quickly and conveniently detect the target molecule L-Trp. The sensor has high sensitivity and advantages. Due to the hydrogen bonding interaction between L-Trp and methacrylic acid and the specific L-Trp molecular recognition process, the hydrogel swells significantly, and the color and L are established by the chromaticity diagram. -Sensors for tryptophan concentration. In addition, the size of the hydrogel particles and the structural stability of the inverse opal hydrogel were studied to compare the sensitivity of the L-Trp sensor.

中文摘要... i
英文摘要... ii
誌謝... iii
目錄... iv
表目錄... vi
圖目錄... vii
第一章 緒論... 1
1.1 前言... 1
1.2 專利檢索... 3
第二章 研究步驟與分析... 6
2.1研究步驟... 6
2.1.1 二氧化矽奈米球之合成... 6
2.1.2 單層二氧化矽結構之模板製作... 6
2.1.3 生物感測器之製備... 6
2.1.3.1 L-Trp預聚合溶液之製備... 6
2.1.3.2 L-Trp水凝膠之製備... 6
2.1.4 實驗分析... 7
第三章 結果與討論... 8
3.1蛋白石與反蛋白石結構分析... 8
3.1.1 二氧化矽奈米球之平均分佈粒徑... 8
3.1.2 二氧化矽奈米球表面結構分析... 8
3.1.2 L-Trp薄膜表面結構分析... 8
3.2生物感測器分析... 8
3.2.1 分子印記水凝膠之檢測曲線... 8
3.2.2 分子印記水凝膠之專一性分析... 8
3.2.3 分子印記水凝膠之濃度分析... 9
3.2.4 分子印記水凝膠之快速響應性分析... 9
3.2.5 分子印記水凝膠之重現性分析... 9
第四章 結論... 10
參考文獻... 11

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