大棘大眼鯛（Priacanthus macracanthus）漁獲量於1996年達最高峰的7,386噸後，即開始逐年減少，2020年時漁獲量僅餘約600噸，為需進行資源復育的目標魚種對象之一。本研究針對大棘大眼鯛育苗技術進行研究，嘗試攻克其初期開口餌料生物問題，並試驗其精子凍結保存條件與可行性實驗，提供未來大棘大眼鯛栽培漁業與資源增裕之魚苗需求。本研究樣本以東港拖網採集，經鏡檢卵徑大於500μm 之雌魚，給予魚用絨毛膜激性腺激素200 IU/kg 1劑後，記錄與量測受精卵與仔魚。精子凍結保存實驗部份，測定精液滲透壓，以4 ℃冷藏條件篩選出適當稀釋液後，再進行冷凍保存試驗。結果顯示，大棘大眼鯛受精卵於水溫28 ℃ 條件下，歷時18小時30分鐘後成功孵化；於水溫26 ℃條件下，歷時20小時58分鐘後成功孵化；而水溫20 ℃ 條件下，歷時30小時30分鐘後成功孵化，水溫愈高仔魚發育速度愈快，且內源性營養利用亦加快，於三個測試水溫（28 ℃、26 ℃、20 ℃）下皆觀察到攝餌仔魚，且28 ℃組於孵化後第5天死亡、26 ℃於孵化後第10天死亡、20 ℃於孵化後第6天死亡。精子凍結保存實驗部份，精液的等滲透壓範圍約283-404 mOsm/kg，稀釋液則以Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12與368 mOsm/kg NaCl Solution擁有較佳精子存活率，並以低倍率稀釋與低抗凍劑濃度為佳。

In 1996, the 6,093t Priacanthus macracanthus (Red bigeye) was caught and reached the highest catch. Soon after, the catch decreased annually, and the catch reduced to 600 t in 2020. It shows that red bigeye is the one of species need to conduct resource restoration. Therefore, in the present study, we focus on the larval rearing of red bigeye, and attempt to overcome the diet for mouth opening. In addition, we tested the sperm cryopreservation condition and the feasibility of red bigeye to benefit cultivating fishery and resource restoration. The present study sample was caught by trawl net (Donggang) and examined by microscope. If the eggs diameter > 500 μm, one dose human chorionic gonadotropin 200 IU/kg would be injected for fish. In addition, the fertilized eggs and the larvae would be measured and recorded by microscope. In the sperm cryopreservation, the sperm osmolality would be measured in advance. The extender was selected by the ability of stored in 4 ℃ used in the experiment of sperm cryopreservation. The results of study showed that the fertilized egg of bigeye took 18 hr 30 min to hatch at 28 ℃. However, it took 30 hr 30 min to hatch at 20 ℃. The higher the water temperature, the faster of larval development and endogenous nutrition utilization, and the feed intake larvae were observed at three water temperature (28 ℃、26 ℃、20 ℃). Also, the larvae dead at 5 days post hatching (dph) in 28 ℃, and the larvae dead at 10 dph in 26 ℃, and the larvae dead at 6 dph in 20 ℃. Result of sperm cryopreservation experiment, the sperm osmolality ranged from 283 - 404 mOsm/kg. Two extender that Dulbecco‘s Modified Eagle Medium/Nutrient Mixture F-12 and 368 mOsm/kg NaCl Solution had good sperm viability. The low dilution and antifreeze concentration had better performance.

摘要 I
ABSTRACT II
謝辭 III
目錄 IV
圖目錄 VII
表目錄 VIII

第壹章 前言 1
第一節 漁業資源與栽培漁業概述 1
第二節 種魚培育 3
第三節 繁殖及育苗 4
第四節 魚類精液凍結保存技術 5
第五節 餌料生物 6
第六節 大棘大眼鯛生態與資源量簡介 7
第七節 研究動機 8
第八節 研究目的與目標 8

第貳章 研究材料與方法 9
第一節 生物數據 9
一 樣本獲取 9
二 生殖腺發育變化 9
第二節 種魚培育 10
一 養殖環境條件準備 10
二 馴化 10
第三節 人工催熟與授精 11
一 卵徑與精子存活率測定 11
二 種魚催熟 11
三 人工授精 11
四 受精卵發育觀察與紀錄 12
第四節 仔魚培育 12
一 育苗環境 12
二 仔稚魚餌料 12
三 餌料培育與滋養 12
四 仔魚觀察 13
第五節 精液冷藏與凍結保存 13
一 樣本取得 13
二 滲透壓與等滲透壓條件測試 13
三 精液冷藏保存試驗 13
四 精液凍結保存試驗 14
五 長期凍結保存試驗 14
六 精子螢光染色判讀 14
第六節 統計分析 15

第參章 結果 16
第一節 東港海域大眼鯛生殖腺年週期性發育變化 16
一 雄、雌魚之GSI變化 16
二 生殖腺發育變化 16
第二節 種魚培育 17
一 野外撈捕 17
二 運輸 17
三 馴化 18
四 生殖腺成熟度檢測 18
第三節 人工催熟與授精 19
一 荷爾蒙刺激與人工授精 19
二 受精卵發育 19
第四節 仔魚培育 21
一 水質變化 21
二 仔魚發育 21
三 仔稚魚餌料 22
第五節 精液凍結保存 23
一 滲透壓與等滲透壓條件 23
二 冷藏保存條件測試 23
三 凍結保存與抗凍劑濃度 24
四 長期凍結保存 24
五 精子螢光染色判讀 25

第肆章 討論 26
第一節 大眼鯛生殖與種魚培育 26
一 繁殖期與生殖 26
二 種魚培育 27
第二節 受精卵與仔魚發育 28
一 受精卵發育 28
第三節 精液凍結保存 30
第伍章 結論與建議 31
參考文獻 32

圖目錄
圖1 1993年至2020年臺灣大眼鯛漁獲量與產值變化 39
圖2 2021年後壁湖每月平均海水表層水溫變化 40
圖3 2021年3月至2022年2月大眼鯛生殖腺指數變化 41
圖4 2021年3月至2022年2月大眼鯛生殖腺外觀變化照片 42
圖5 2021年3月至2022年2月大眼鯛鏡檢卵細胞發育變化照片 43
圖6 2021年3月至2022年2月間大眼鯛之不同大小卵細胞佔比頻度 44
圖7 2021年3月至2022年2月大眼鯛精巢組織切片變化照片 45
圖8 2021年3月至2022年2月大眼鯛卵巢組織切片變化照片 46
圖9 大眼鯛運輸流程照片 47
圖10 大眼鯛生殖腺檢測照片 48
圖11 大眼鯛注射激素催熟照片 49
圖12 大眼鯛人工授精流程照片 50
圖13 大眼鯛受精卵於不同水溫發育過程照片 51
圖14 大眼鯛仔魚在不同水溫發育過程照片 52
圖15 大眼鯛仔魚在不同水溫成長變化 53
圖16 大眼鯛仔魚在不同水溫攝餌照片 54
圖17 大眼鯛精液分別添加3種稀釋液與使用4種稀釋比例之精子存活率變化 55
圖18 大眼鯛稀釋精液以4種濃度分別添加3種抗凍劑之精子存活率變化 56
圖19 大眼鯛稀釋精液於長期凍結保存之精子存活率變化 57
圖20 精子螢光染色照片 58
圖21 螢光染色判讀新鮮與凍結24小時精液之精子存活率變化 59

表目錄
表1 受精卵發育特徵 60
表2 仔魚養殖期間水質變化 61

中文文獻
朱惠真、陳力豪、胡鄴方、蔡惠萍、張錦宜，2015。生殖細胞冷凍保存技術的現況。水試專訊。50，48-50。
吳春基、吳龍靜，2016。臺灣西南海域拖網漁業之重要漁獲魚類。水產試驗所特刊。20，131。
宋嘉軒，2018。淺談近交衰退。水試專訊。64，55-56。
翁進興、黃建智、吳春基、劉光明、吳龍靜，2017。臺灣西南海域大棘大眼鯛之生殖生態研究。水產研究。25，1-13。
區又君、謝木嬌、李加兒、溫久福、周慧、劉奇奇，2017。廣東池塘培育四指馬鮁親魚初次性成熟和苗種規模化繁育技術研究。南方水產科學。13，98-104。
張賜玲、鄭明忠、廖紹文、劉世傑、李彥宏、陳紫媖，2008。大眼鯛的繁養殖初報。水試專訊。22，6-8。
陳玉萍、陳鏗元、劉恩良、何源興，2017。牙鮃的誘導產卵及幼苗發育。水產研究。25，43-53。
陳松林，2002。魚類配子和胚胎冷凍保存研究進展及前景展望。水產學報。26，161-168。
蔡惠萍、吳允暉、何源興、張錦宜，2016。臺灣東部海域赤鯥精液冷凍保存技術之初步探討。水試專訊。55，12-15。

外文文獻
Agarwal, N.K., 2011. Cryopreservation of Fish Semen. Himalayan Aquatic Biodiversity Conservation & New Tools in Biotechnology. 104-127.
Allendorf, F.W., Leary, R.F., Spruell, P., Wenburg, J.K., 2011. The problems with hybrids: setting conservation guidelines. Trends in Ecology & Evolution. 16, 613-622.
Balamurugan, R., Munuswamy, N., 2017. Cryopreservation of sperm in Grey mullet Mugil cephalus (Linnaeus,1758). Animal Reproduction Science. 185, 205-213.
Bang, M., Kang, S., Kim, S., Jang, C.J., 2018. Changes in the Biological Characteristics of Walleye Pollock Related to Demographic Changes in the East Sea During the Late 20th Century. Marine and Coastal Fisheries. 10, 91-99.
Baskerville-Bridges, B., Kling, L.J., 2000. Early weaning of Atlantic cod (Gadus morhua) larvae onto a microparticulate diet. Aquaculture. 189, 109-117.
Best, B.P., 2015. Cryoprotectant Toxicity: Facts, Issues, and Questions. Rejuvenation Research. 18, 422-436.
Cahu, C., Infante, J.Z., 2001. Substitution of live food by formulated diets in marine fish larvae. Aquaculture. 200, 161-180.
Carić, M., Sanko-Njire, J., Skaramuca, B., 1993. Dietary effects of different feeds on the biochemical composition of the rotifer (Brachionus plicatilis Müller). Aquaculture. 110, 141-150.
Chao, N.H., Chao, W.C., Liu, K.C., Liao, I.C., 1987. The properties of tilapia sperm and its cryopreservation. Journal of Fish Biology. 30, 107-118.
Christian, F., Maec, S., Armelle, S., Constantinos C.M., Nikos, P., 2008. Slow-release GnRHa treatment prevented atresia during vitellogenesis and induced ovulation of captive wreckfish (Polyprion americanus). International Journal of Ichthyology. 32, p.191.
Contreras, P., Dumorné, K., Ulloa-Rodríguez, P., Merino, O., Figueroa, E., Farías, JG., Valdebenito, I., Risopatrón, J., 2019. Effects of short-term storage on sperm function in fish semen: a review. Reviews in Aquaculture. 12, 1373-1389.
COWX, I.G., 1994. Stocking strategies. Fisheries Management and Ecology. 1, 15-30.
Day, J.C., Dobbs, K., 2013. Effective governance of a large and complex cross-jurisdictional marine protected area: Australia’s Great Barrier Reef. Marine Policy. 41, 14-24.
Di lorio, M., Esposito, S., Rusco, G., Roncarati, A., Miranda, M., Gibertoni, P.P., Cerolini, S., Iaffaldano, N., 2019. Semen cryopreservation for the Mediterranean brown trout of the Biferno River (Molise-Italy): comparative study on the effects of basic extenders and cryoprotectants. Scientific Reports. 9, 9703.
Falcón, J., Besseau, L., Sauzet, S., Boeuf, G., 2007. Melatonin effects on the hypothalamo-pituitary axis in fish. Trends in Endocrinology & Metabolism. 18, 81-88.
Falcón, J., Migaud, H., Muñoz-Cueto, J.A., Carrillo, M., 2010. Current knowledge on the melatonin system in teleost fish. General and Comparative Endocrinology. 165, 469-482.
FAO, 2018. The State of World Fisheries and Aquaculture 2018. Rome, 210.
FAO, 2020. The State of World Fisheries and Aquaculture 2020. Rome, 206.
Fraser, K.A., Adams, V.M., Pressey, R.L., Pandolfi, J.M., 2019. Impact evaluation and conservation outcomes in marine protected areas：A case study of the Great Barrier Reef Marine Park. Biological Conservation. 238, 1-13.
Fraser, T.W.K., Hansen, T., Fleming, M.S., Fjelldal P.G., 2013. The prevalence of vertebral deformities is increased with higher egg incubation temperatures and triploidy in Atlantic salmon Salmo salar L. Journal of Fish Diseases. 38, 75-89.
Han, Y.S., Liao, I.C., Huang, Y.S., He, J.T., Chang, C.W., Tzeng, W.N., 2003. Synchronous changes of morphology and gonadal development of silvering Japanese eel Anguilla japonica. Aquaculture. 219, 783-796.
Hershberger, W.K., Myers, J.M., Iwamoto, R.N., Mcauley, W.C., Saxton, A.M., 1990. Genetic Changes in the Growth of Coho Salmon (Oncorhynchus kisutch) in Marine Net-Pens, Produced by Ten Years of Selection. Aquaculture. 85, 187-197.
Hilario, M., Fran, S.R., 2003. Female Reproductive Strategies of Marine Fish Species of the North Atlantic. Journal of Northwest Atlantic Fishery Science. 33, 23-31.
Hines, C.W., 2019. The effect of salinity and photoperiod on thermal tolerance and growth of Atlantic and coho salmon reared from smolt to adult in recirculating aquaculture systems. The University of British Columbia. Thesis, 69.
Ikusô, H., Kenichiro, K., Tetsuichiro, K., 1971. Effect of temperature on the body from and mortality in the developmental and early larval stages of the Alaska pollack, Theragra chalcogramma (pallas). Bulletin of Fisheries Sciences. 22, 11-29.
Jabbar, M.A., Kamal, M.M., Boer, M., Suman, A., Suyasa, I.N., 2018. Reproductive biology of the red bigeye (Priacanthus macracanthus; Cuvier, 1829) in Palabuhanratu bay, Indonesia. Indonesian Fisheries Research Journal. 24, 11-22.
Joo-young, S., Kwon, O.N., 2017. Naturally Collection and Development until Yolk Absorption of Domestic Walleye Pollock Theragra chalcogramma Fertilized Eggs and Larvae. Journal of the Korea Academia-Industrial cooperation Society. 18, 49-54.
Joung, S.J., Chen, C.T., 1992. Age and Growth of the Big Eye Priacanthus macracanthus from the Surrounding Water of Guei-Shan Island, Taiwan. Nippon Suisan Gakkaishi. 58, 481-488.
Kang, S., Kim, S., 2015. What caused the collapse of walleye pollock population in Korean waters?. KMI International Journal of Maritime Affairs and Fisheries. 7, 43-58.
Kim, D.H., Kang, S., Kim, S., 2014. Market and consumption of walleye pollock in Korea. Fisheries Science. 80, 213-218.
Kolkovski, S., Lazo, J., Leclercq, D., Izquierdo, M., 2009. Fish larvae nutrition and diet: new developments. New Technologies in Aquaculture. Woodhead, 315-369.
Laikre, L., Schwartz, M.K., Waples, RS., Ryman, N., 2010. Compromising genetic diversity in the wild: unmonitored large-scale release of plants and animals. Trends in Ecology & Evolution. 25, 520-529.
Lester, R. J.G., Watson R.A., 1985. Growth, mortality, parasitism, and potential yields of two Priacanthus species in the South China Sea. Journal of Fish Biology. 27, 307-318.
Leung, L.KP., 1987. Cryopreservation of spermatozoa of the barramundi, Lates calcarifer (Teleostei: Centropomidae). Aquaculture. 64, 243-247.
Lie, O., Haaland, H., Hemre, G.I., Maage, A., Lied, E., Rosenlund, G., Sandnes, K., Olsen, Y., 1997. Nutritional composition of rotifers following a change in diet from yeast and emulsified oil to microalgae. Aquaculture International 5, 427-438.
Liu, K.M., Chen, C.T., Yang, R.H., 1999. Estimates of Age and Growth on the Big Eye Priacanthus macracanthus in the Northeastern Taiwan Waters. Fisheries Science. 65, 211-217.
Liu, K.M., Hung, K.Y., Chen, C.T., 2001. Reproductive biology of the big eye Priacanthus macracanthus in the north-eastern waters off Taiwan. Fisheries Science. 67, 1008-1014.
Liu, Q.H., Ma, D.Y., Xu, S.H., Xiao, Z.Z., Xiao, Y.S., Song, Z.C., Li, J., 2015. Summer flounder (Paralichthys dentatus) sperm cryopreservation and application in interspecific hybridization with olive flounder (P olivaceus). Theriogenology. 83, 703-710.
Matsui, H., Shiozaki, K., Okumura, Y., Ishikawa, M., Waqalevu, V., Hayasaka, O., Honda, A., Kotani, T., 2020. Effects of phosphorous deficiency of a microalga Nannochloropsis oculata on its fatty acid profiles and intracellular structure and the effectiveness in rotifer nutrition. Algal Research. 49, 101905.
Mayer, I., Shackley, S.E., Witthames, P.R., 1990. Aspects of the reproductive biology of the bass, Dicentrarchus labrax L. II. Fecundity and pattern of oocyte development. Journal of Fish Biology. 36, 141-148.
Maynard, D.J., Trial, J.G., 2014. The use of hatchery technology for the conservation of Pacific and Atlantic salmon. Reviews in Fish Biology and Fisheries. 24, 803-817.
McBride, R.S., Somarakis, S., Fitzhugh, G.R., Albert, A., Yaragina, N.A., Wuenschel, M.J., Alonso-Fernández, A., Basilone, G., 2013. Energy acquisition and allocation to egg production in relation to fish reproductive strategies. Fish and Fisheries. 16, 23-57.
Moorhead, J.A., Zeng, C., 2010. Development of Captive Breeding Techniques for Marine Ornamental Fish: A Review. Reviews in Fisheries Science. 18, 315-343.
Muchlisin, Z.A., 2005. REVIEW:Current Status of Extenders and Cryoprotectants on FishSpermatozoa Cryopreservation. Biodiversita. 6, 66-69.
Murua, H., Motos, L., 2006. Reproductive strategy and spawning activity of the European hake Merluccius merluccius (L.) in the Bay of Biscay. Journal of Fish Biology. 69, 1288-1303.
Mylonas, C.C., Fostier, A., Zanuy, S., 2010. Broodstock management and hormonal manipulations of fish reproduction. General and Comparative Endocrinology. 165, 516-534.
Nardi, G., Prickett, R., Meeren T., Boyce, D., Moir, J., 2021. Atlantic cod aquaculture: Boom, bust, and rebirth?. Journal of the World Aquaculture Society. 52, 672-690.
Neira, R., Lhorente, J.P., Newman, S., Yáñez, J.M., Araneda, M., Filp, M., 2014. Evolution of Coho Salmon (Oncorhynchus kisutch) Breeding Programs. 10th World Congress of Genetics Applied to Livestock Production. Vancouver, 6.
Oki, D., Taberta, O., 1999. Reproductive Characteristics of Big Eye Priacanthus macracanthus in the East China Sea. Fisheries Science. 65, 835-838.
Ørnsrud, R., Gil, L., Waagbø, R., 2004. Teratogenicity of elevated egg incubation temperature and egg vitamin A status in Atlantic salmon, Salmo salar L. Journal of Fish Diseases. 27, 213-223.
Öztürk, A. E., Bucak, M. N., Bodu, M., Başpınar, N., Çelik, İ., Shu, Z., Kekin, N., Gao, D., 2019. Cryobiology and cryopreservation of sperm. In Cryopreservation-Current Advances and Evaluations. IntechOpen. 190.
Peña, R., Dumas, S., Zavala-Leal, I., Contreras-Olguín, M., 2012. Effect of incubation temperature on the embryonic development and yolk-sac larvae of the Pacific red snapper Lutjanus peru (Nichols & Murphy, 1922). Aquaculture Research. 45, 519-527.
Reisenbichler, R.R., Rubin, S.P., 1999. Genetic changes from artificial propagation of Pacific salmon affect the productivity and viability of supplemented populations. ICES Journal of Marine Science. 56, 459-466.
Ruyet, J.P.L., Alexandre, J.C., Thébaud, L., Mugnier, C., 1993. Marine Fish Larvae Feeding: Formulated Diets or Live Prey?. Journal of the World Aquaculture Society. 24, 211-224.
Schreck, C.B., Contreras-Sanchez, W.C., Fitzpatrick, M.S., 2001. Effects of stress on fish reproduction, gamete quality, and progeny. Reproductive Biotechnology in Finfish Aquaculture. 3-24.
Varkentin, A.I., Sergeeva, N.P., 2004. The fisheries and current state of walleye pollock (Theragra chalcogramma) stock abundance in the eastern Sea of Okhotsk. PICES Scientific Report. 26, 251.
Walford, J., Lim, T.M., Lam, T.J., 1991. Replacing live foods with microencapsulated diets in the rearing of seabass (Lates calcarifer) larvae: do the larvae ingest and digest protein-membrane microcapsules?. Aquaculture. 92, 225-235.
Watanabe, W.O., Lee, C.S., Ellis, S.C., Ellis, EP., 1995. Hatchery study of the effects of temperature on eggs and yolksac larvae of the Nassau grouper Epinephelus striatus. Aquaculture. 136, 141-147.
Xin, M., Siddique, M.A.M., Dzyuba, B., Cuevas-Uribe, R., Shaliutina- Kolešová, A., Linhart, O., 2017. Progress and challenges of fish sperm vitrification: A mini review. Theriogenology. 98, 16-22.
Young, K. A., 2013. The balancing act of captive breeding programmes: salmon stocking and angler catch statistics. Fisheries Management and Ecology. 20, 434-444.
Yúfera, M., Darias, M.J., 2007. The onset of exogenous feeding in marine fish larvae. Aquaculture. 268, 53-63.
八木佑太、新田誠、飯田直樹、竹内宏行、山田達哉、長副聡、山本岳男、高原英生、井関智明、上原伸二, 2015。アカムツの卵発生と仔魚の形態。魚類学雑誌。62, 163-168。
荒井大介, 2013。栽培漁業の現場における遺伝的多様性を確保する取り組みの現状。日本水産学会誌。79, p.83。
飯田直樹, 2016。アカムツ稚魚放流しました～輸送・放流方法の検討～。富山県農林水産総合技術センター水産研究所。17, 2-3。

網路文獻
中央氣象局臺灣海象災防環境資訊平台 (2021)。2021年潮位站觀測歷史資料。2021年9月12日，取自: https://ocean.cwb.gov.tw/V2/data_interface/download?dataset=Tide-his&title=%E6%BD%AE%E4%BD%8D%E7%AB%99%E8%A7%80%E6%B8%AC%E6%AD%B7%E5%8F%B2%E8%B3%87%E6%96%99&format=CSV&all_formats=TXT,CSV&token=eyJ0eXAiOiJKV1QiLCJhbGciOiJIUzI1NiJ9.eyJkYXRhc2V0SUQiOiIxNDEiLCJ0IjoxNjYxMTQ3OTExfQ.9N1RrM234xmGp_sYVXYktF96qug8bEKSM-zjo7fLLsM
行政院農業委員會漁業署 (2021)。中華民國臺閩地區漁業統計年報。2021年11月7日，取自: https://www.fa.gov.tw/list.php?theme=FS_AR&subtheme=&page=1&
富山県農林水産総合技術センター水産研究所 (2013)。No.636-1:国内初、アカムツ(ノドグロ)の稚魚生産に成功。2021年11月3日，取自: https://www.toyama-brand.jp/TJN/?tid=103679
Ministry of Oceans and Fisheries (2014)。국민식탁에 동해산 우리 명태 올린다。2021年6月5日，取自: https://www.mof.go.kr/article/view.do?articleKey=1684&searchSelect=title&boardKey=10&menuKey=376¤tPageNo=390
Ministry of Oceans and Fisheries (2015)。동해 명태 살리기 위해 여의도 면적 7.4배 보호수면 지정。2021年6月5日，取自: https://www.mof.go.kr/article/view.do?articleKey=9561&searchSelect=title&boardKey=10&menuKey=376¤tPageNo=233
Ministry of Oceans and Fisheries (2016)。세계 최초로 명태 '완전양식 기술' 개발 성공。2021年6月5日，取自: https://www.mof.go.kr/article/view.do?articleKey=13565&searchSelect=title&boardKey=10&menuKey=376¤tPageNo=112
National Oceanic and Atmospheric Administration (2022). Cold & Warm Episodes by Season (1950 – 2022). Retrieved August 22, 2022, from: https://origin.cpc.ncep.noaa.gov/products/analysis_monitoring/ensostuff/ONI_v5.php