臺灣博碩士論文加值系統

本研究是探究光電動微流體技術對細胞操控速度與存活率的影響。利用波長為1064 nm的近紅外光雷射和交流電場在微流體晶片中產生微渦流，從而使細胞流向雷射中心處並聚集於基板表面。首先，我們使用多重物理量耦合分析軟體(COMSOL Multiphysics 5.5a)對微流道進行了光電動模擬分析，包括溫度梯度、電場梯度、熱泳力、介電泳力、電滲流、拖曳力、聚集力以及細胞速度等。這些模擬結果為我們進一步研究光電動微流體技術對細胞行為的影響提供了理論基礎。微流道晶片的製作採用氧化銦錫(ITO)玻璃作為上板與基板，中間層使用聚對苯二甲酸乙二酯(PET)薄膜厚度為75 μm的光學膠帶製作出微流道的高度。在實驗上利用254 W/cm2的雷射光和施加4 VPP及20 kHz的交流電場，可以使平均直徑約7.5 μm的紅血球在微流道中達到2.8 μm/s的速度移動並朝向雷射中心處聚集，並利用紅血球解凍後的時間差異來分析得知操控速度隨時間下降。另外利用445 W/cm2的雷射光和施加20 VPP及20 kHz的交流電場，可以聚集平均直徑約18 μm的K562細胞，使其達到1.4 μm/s的速度移動並朝向雷射中心處聚集，結果顯示K562細胞在存活率遞減下的收集速度呈現下降趨勢，實驗證明光電動微流體技術可對細胞進行操控，並可以由操控速度得知細胞會隨著存活率遞減而下降。

The objective of this study is to investigate the effect of optoelectrokinetic microfluidics on cell manipulation velocity and viability. Microvortices are generated in the microfluidic chip using a near-infrared laser with a wavelength of 1,064 nm and an AC electric field. This causes cells to flow towards the center of the laser and collect on the surface of the substrate. First of all, the optoelectrokinetic simulation of microchannels is performed using COMSOL Multiphysics software. This includes analyzing the effects of temperature gradient, electric field gradient, thermophoretic force, dielectrophoresis force, electroosmotic flow, drag force, trapping force, and cell velocity. The simulation results provide a theoretical foundation for further investigations into the impact of optoelectrokinetic microfluidics on cell behavior. Indium tin oxide (ITO) glass is utilized as the top plate and substrate during the construction of the microfluidic chip. Films constructed of polyethylene terephthalate (PET) have a thickness of 75 μm and make up the intermediate layer. In the test, red blood cells with an average diameter of about 7.5 μm were observed to move at a velocity of 2.8 μm/s in the microchannel. They collected towards the center of the laser beam after being exposed to a laser power of 254 W/cm2. Additionally, they were placed in an AC electric field of 4 VPP and 20 kHz. The time difference of red blood cells after thawing was analyzed. The analysis results show that the velocity of manipulation decreases over time. In addition, K562 cells with an average diameter of about 18 μm can move at a velocity of 1.4 μm/s and collect towards the center of the laser after being exposed to a laser beam with a power density of 445 W/cm2. They are then placed in an AC electric field with an amplitude of 20 VPP and a frequency of 20 kHz. The results show that the collection velocity of K562 cells decreases as their viability decreases. The test demonstrates that optoelectrokinetic microfluidics can manipulate cells. It can be observed from the manipulation velocity that the collection velocity of cells decreases as the viability decreases.

摘要………………………………………………………………………i
Abstract…………………………………………………………………ii
誌謝………………………………………………………………iv
目錄……………………………………………………………………v
表目錄…………………………………………………………………viii
圖目錄………………………………………………………………ix
符號說明……………………………………………………………xiii
第一章 緒論……………………………………………………………1
1.1 前言……………………………………………………………1
1.2 研究動機與目的………………………………………………1
1.3 文獻回顧………………………………………………………1
1.3.1 介電泳技術………………………………………………1
1.3.2 光電動技術…………………………………………………3
1.4 論文架構………………………………………………………7
第二章 系統架構介紹…………………………………………………8
2.1 系統設備………………………………………………………8
2.2 晶片材料及粒子溶液…………………………………13
第三章 光電動力學…………………………………………………17
3.1 電泳(Electrophoresis)…………………………………17
3.2 電偶極(Dipole)…………………………………17
3.3 介電泳(Dielectrophoresis, DEP)…………………………………20
3.4 電滲流(Electroosmosis flow, EOF)…………………………………21
3.5 交流電熱(AC Electrothermal, ACET)…………………………………21
3.6 熱泳(Thermophoresis)…………………………………22
3.7 細胞計數(cell counts)…………………………………23
第四章 數值分析與晶片設計………………………………………24
4.1 模型建模……………………………………………………25
4.2 溫度梯度……………………………………………………26
4.3 電場梯度……………………………………………………27
4.4 電滲流………………………………………………………28
4.5 環形流場……………………………………………………29
4.6 介電泳力……………………………………………………30
4.7 熱泳力………………………………………………………31
4.8 拖曳力………………………………………………………32
4.9 聚集力…………………………………………………………33
4.10 粒子速度……………………………………………………34
4.11 晶片設計與實驗架構…………………………………………35
第五章 實驗結果與討論……………………………………………37
5.1 粒子受力分析…………………………………37
5.2 紅血球的操控與速度分析…………………………………38
5.2.1 紅血球第1天操控距離及操控速度與時間…………………………………38
5.2.2 紅血球第3天操控距離及操控速度與時間…………………………………40
5.2.3 紅血球第1天與第3天操控比較…………………………………41
5.3 K562細胞的操控與存活率 43
5.3.1 K562細胞第1天操控距離及其操控速度、時間與活性關係……………………………………………………………43
5.3.2 K562細胞第2天操控距離操控速度、時間與活性……………………………………………………………45
5.3.3 K562細胞第3天操控距離及其操控速度、時間與活性…………………………………………………………47
5.3.4 K562細胞第1天、第2天與第3天操控距離操控速度、時間與活性比較………………………………………………50
第六章 結論與未來展望……………………………………………51
6.1 結論…………………………………51
6.2 未來展望…………………………………52
參考文獻………………………………………………………………53
Extended Abstract………………………………………………………59

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