目錄
壹、前言 ...................................................................1
貳、文獻回顧 ...............................................................2
一、糖尿病與慢性傷口 ....................................................... 2
二、慢性傷口照護 ......... ................................................. 4
三、巨噬細胞在傷口癒合中的角色與功能...........................................6
四、高血糖對巨噬細胞之影響 ...................................................7
五、膠原蛋白對傷口修復的影響 .................................................8
(一)陸生與海洋膠原蛋白 ..................................................... 9
(二)吳郭魚皮第一型膠原蛋白 (tilapia skin collagen, TSC) .................... 10
(三)鱘龍魚軟骨第二型膠原蛋白 ( sturgeon cartilage collagen, SCC ) ........... 11
六、傷口癒合機制 ........................................................... 12
參、研究目的................................................................ 17
肆、材料與方法.............................................................. 18
一、實驗材料 ............................................................... 18
二、儀器與廠牌 ............................................................. 19
三、藥品 ....................................................................20
四、實驗方法 ............................................................... 21
(一)實驗架構 ............................................................... 21
(二)膠原蛋白之製備 .......................................................... 22
(三)膠原蛋白之 coating ...................................................... 22
(四)細胞培養 - RAW264.7 小鼠巨噬細胞 ......................................... 23
(五)細胞增生分析 ( trypan blue assay ) ...................................... 23
(六)細胞劃傷試驗 ( scratch assay ) .......................................... 24
(七)動物實驗 (in vivo study) ................................................ 25
(八)皮膚病理組織切片 ......................................................... 26
(九)統計分析 (statistical analysis) ......................................... 30
伍、結果與討論................................................................ 31
一、 TSC 與 SCC 對傷口癒合之體外實驗評估....................................... 31
(一) TSC 與 SCC 對 RAW264.7 巨噬細胞增生能力之影響 ............................ 31
(二) TSC 與 SCC 加速 RAW264.7 巨噬細胞之遷移能力 .............................. 33
二、 TSC 與 SCC 對糖尿病傷口癒合之體內實驗評估 ................................. 34
(一) 以STZ誘導糖尿病對C57BL/6小鼠血糖變化之影響 ................................ 34
(二) TSC 與 SCC 促進 C57BL/6 糖尿病小鼠皮膚傷口修復 ........................... 35
陸、結論 .................................................................... 44
柒、圖表 .................................................................... 45
捌、參考文獻 ................................................................. 70

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