臺灣博碩士論文加值系統

本實驗在室內循環水養殖系統中，將300公克±1公克的筍殼魚 (marble goby, 學名 Oxyeleotris marmorata)進行了150天的試驗，探討飼料以不同比例豆粕 (soybean meal, SM) 替代原有飼料中的魚粉(fish meal,FM)，並添加1%牛磺酸(taurine,t)來探討對筍殼魚生長影響。8種飼料配方以SM代替20%、35% 或 50%的魚粉與加入牛磺酸：FM、FM-t、SM20、SM20-t、SM35、SM35-t、SM50和SM50-t。並在90、105、120、135以及150天紀錄魚體各種生長參數。實驗結果顯示，SM20-t飼料的對於的生長，飼料轉換率以及飼料採食率最接近FM飼料組。如此 飼養期間的飼料性能且降低成本，且飼料添加牛磺酸可以明顯著提高筍殼魚的生長率和飼料利用率。

In this experiment, an indoor circulation aquaculture system, marble goby (Oxyeleotris marmorata) was tested for 150 days to explore the use of different proportions of soybean meal (soybean meal, SM) to replace the fish meal in the original feed (fish meal, FM), and add 1% taurine (taurine, t) to investigate the effects on the growth of marble goby. Eight feed formulations replaced 20%, 35% or 50% of fish meal with SM and added taurine, named FM, FM-t, SM20, SM20-t, SM35, SM35-t, SM50 and SM50-t. Various growth parameters of experiment fish at 90, 105, 120, 135 and 150 days were recorded. The experimental results showed that the growth, feed conversion rate and feed intake rate of the SM20-t diet were the closest to the FM diet group. In this way, the feed performance during the feeding period was improved and the feed cost was reduced. Moreover, the growth and feed utilization rate of the marble goby in the study were significantly improved by addition of taurine to the formulated feed.

致謝 i
摘要 ii
Abstract iii
目錄 iv
表目錄 vi
圖目錄 viii
壹、前言 1
貳、文獻回顧 2
一、筍殼魚 2
二、魚粉 5
三、豆粕 6
四、牛磺酸 8
五、室內循環水養殖 11
參、實驗方法與材料 14
一、實驗材料 14
二、實驗材料與器材 17
三、實驗架構 18
四、實驗飼料設計 19
五、魚和水池 22
六、樣本採集及分析 26
七、統計分析 31
肆、實驗結果與討論 32
一、結果討論 32
二、不同飼料配方對於魚的體重增加結果 34
三、不同飼料配方對於魚的採食量差別 45
四、不同飼料配方對於魚的飼料轉化效率差別 56
五、具體增長率 67
六、不同飼料配方對於魚的肝重指數(HSI)以及內臟指數(VSI) 78
伍、結論 81
陸、參考文獻 82

Aaen, S. M., Helgesen, K. O., Bakke, M. J., Kaur, K., & Horsberg, T. E. (2015). Drug resistance in sea lice: a threat to salmonid aquaculture. Trends in Parasitology, 31(2), 72-81.
Brosnan, J. T., & Brosnan, M. E. (2006). The sulfur-containing amino acids: an overview. J Nutr, 136(6 Suppl), 1636s-1640s.
Chatzifotis, S., Polemitou, I., Divanach, P., & Antonopoulou, E. (2008). Effect of dietary taurine supplementation on growth performance and bile salt activated lipase activity of common dentex, Dentex dentex, fed a fish meal/soy protein concentrate-based diet. Aquaculture, 275(1), 201-208.
Francis, G., Makkar, H., & Becker, K. (2001). Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture, 199, 197-227.
Galindo-Villegas, J., García-Alcazar, A., Meseguer, J., & Mulero, V. (2019). Aluminum adjuvant potentiates gilthead seabream immune responses but induces toxicity in splenic melanomacrophage centers. Fish & Shellfish Immunology, 85, 31-43.
Gunathilaka, G. L. B. E., Kim, M.-G., Lee, C., Shin, J., Lee, B.-J., & Lee, K.-J. (2019). Effects of taurine supplementation in low fish meal diets for red seabream (Pagrus major) in low water temperature season. Fisheries and Aquatic Sciences, 22(1), 23.
Herman, E. M., & Schmidt, M. A. (2016). The Potential for Engineering Enhanced Functional-Feed Soybeans for Sustainable Aquaculture Feed. Front Plant Sci, 7, 440. doi:10.3389/fpls.2016.00440
Krishnan, H. B., & Jez, J. M. (2018). Review: The promise and limits for enhancing sulfur-containing amino acid content of soybean seed. Plant Science, 272, 14-21.
Krogdahl, A., Kortner, T. M., Jaramillo-Torres, A., Gamil, A. A. A., Chikwati, E., Li, Y., . . . Storebakken, T. (2020). Removal of three proteinaceous antinutrients from soybean does not mitigate soybean-induced enteritis in Atlantic salmon (Salmo salar, L). Aquaculture, 514, 734495.
Larson H.K. & Murdy E.O., 2001.-Families Eleotridae, gobiidae. In:
Species identification Guide for Fishery Purposes. Bony Fishes Part
4., vol. 6 (Carpenter K.E. & Niem v.H., eds), pp. 3574-3603. Rome:
Fao.
Li, M., Lai, H., Li, Q., Gong, S., & Wang, R. (2016). Effects of dietary taurine on growth, immunity and hyperammonemia in juvenile yellow catfish Pelteobagrus fulvidraco fed all-plant protein diets. Aquaculture, 450, 349-355.
Lim, S.J., Oh, D.H., Khosravi, S., Cha, J.H., Park, S.H., Kim, K.W., & Lee, K.J. (2013). Taurine is an essential nutrient for juvenile parrot fish Oplegnathus fasciatus. Aquaculture, 414-415, 274-279.
Marcinkiewicz, J., & Kontny, E. (2014). Taurine and inflammatory diseases. Amino Acids, 46(1), 7-20. doi:10.1007/s00726-012-1361-4
Martinez, J. B., Chatzifotis, S., Divanach, P., & Takeuchi, T. (2004). Effect of dietary taurine supplementation on growth performance and feed selection of sea bass Dicentrarchus labrax fry fed with demand-feeders. Fisheries Science, 70, 74-79.
Martins, N., Estevão-Rodrigues, T., Diógenes, A. F., Diaz-Rosales, P., Oliva-Teles, A., & Peres, H. (2018). Taurine requirement for growth and nitrogen accretion of European sea bass (Dicentrarchus labrax, L.) juveniles. Aquaculture, 494, 19-25.
Matsunari, H., Hamada, K., Mushiake, K., & Takeuchi, T. (2006). Effects of taurine levels in broodstock diet on reproductive performance of yellowtail Seriola quinqueradiata. Fisheries Science, 72(5), 955-960. doi:10.1111/j.1444-2906.2006.01243.x
Miles, R. D., & Chapman, F. A. (2012). The Benefits of Fish Meal in Aquaculture Diets 1.
Mukherjee, R., Chakraborty, R., Dutta, A., 2016. Role of fermentation in
improving nutritional quality of soybean meal—a review.
Asian-Australasian. J. Anim. Sci. 29, 1523.
Nguyen, H. P., Khaoian, P., Fukada, H., Suzuki, N., & Masumoto, T. (2015). Feeding fermented soybean meal diet supplemented with taurine to yellowtail Seriola quinqueradiata affects growth performance and lipid digestion. Aquaculture Research, 46, 1101-1110.
Nguyen, H. P., Khaoian, P., Furutani, T., Nagano, J., Fukada, H., & Masumoto, T. (2017). Effects of alcohol extract of defatted soybean meal on growth performance and digestive physiology of yellowtail Seriola quinqueradiata. Fisheries Science, 83(1), 99-106. doi:10.1007/s12562-016-1049-x
Ripps, H., & Shen, W. (2012). Review: taurine: a "very essential" amino acid. Mol Vis, 18, 2673-2686.
Salze, G. P., & Davis, D. A. (2015). Taurine: a critical nutrient for future fish feeds. Aquaculture, 437, 215-229.
Schaffer, S. W., Ju Jong, C., Kc, R., & Azuma, J. (2010). Physiological roles of taurine in heart and muscle. Journal of Biomedical Science, 17(1), S2. doi:10.1186/1423-0127-17-S1-S2
Silva-Carrillo, Y., Hernández, C., Hardy, R. W., González-Rodríguez, B., & Castillo-Vargasmachuca, S. (2012). The effect of substituting fish meal with soybean meal on growth, feed efficiency, body composition and blood chemistry in juvenile spotted rose snapper Lutjanus guttatus (Steindachner, 1869). Aquaculture, 364-365, 180-185.
Tacon, A. G. J., & Metian, M. (2015). Feed Matters: Satisfying the Feed Demand of Aquaculture. Reviews in Fisheries Science & Aquaculture, 23(1), 1-10.
Takagi, S., Murata, H., Goto, T., Endo, M., Yamashita, H., & Ukawa, M. (2008). Taurine is an essential nutrient for yellowtail Seriola quinqueradiata fed non-fish meal diets based on soy protein concentrate. Aquaculture, 280(1), 198-205.
Teles, A. O., Couto, A., Enes, P., & Peres, H. (2019). Dietary protein requirements of fish – a meta‐analysis. Reviews in Aquaculture.
Wang, L., Zhou, H., He, R., Xu, W., & He, G. (2016). Effects of soybean meal fermentation by Lactobacillus plantarum P8 on growth, immune responses, and intestinal morphology in juvenile turbot (Scophthalmus maximus L.). Aquaculture, 464, 87-94.
Wei, Y., Liang, M., Xu, H., & Zheng, K. (2019). Taurine alone or in combination with fish protein hydrolysate affects growth performance, taurine transport and metabolism in juvenile turbot (Scophthalmus maximus L.). Aquaculture Nutrition, 25(2), 396-405.
Zhang, C. G., & Kim, S. J. (2007). Taurine induces anti-anxiety by activating strychnine-sensitive glycine receptor in vivo. Ann Nutr Metab, 51(4), 379-386.
伍漢霖, 鐘俊生(2008) 中國動物誌-硬骨魚綱-鱸形目（五）-鰕虎魚
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