臺灣博碩士論文加值系統

摘 要 i
Abstract iii
目錄 v
表目錄 vii
圖目錄 viii
附圖目錄 ix
第一章 前言 1
一、 石化塑膠與環境污染 1
二、 聚羥基烷酸酯 (polyhydroxyalkanoates、PHA) 1
1. 介紹 1
2. 化學結構與物理性質 2
3. 生合成路徑 3
4. PHA的應用 4
三、 果糖酸 (levulinic acid, LA) 5
四、 葡萄糖酸 (gluconic acid, GA) 6
五、 共聚物類型 6
六、 研究目的與策略 7
第二章 材料方法 9
一、 實驗菌株及培養基 9
二、 實驗藥品 9
三、 實驗儀器 9
四、 PHA的累積試驗 9
五、 PHA的循環培養試驗 (cyclic fermentation) 10
六、 饋料批式醱酵 (fed-batch fermentation) 試驗 10
七、 Cupriavidus sp. L7L ΔwcaJ比生長速率的測定 11
八、 PHA含量與單體組成分析 12
1. 轉酯化反應 12
2. 樣品製備及氣相層析儀分析 12
九、 PHA的萃取 12
十、 PHA聚合物化學結構之分析 13
十一、 PHA聚合物熱性質分析 13
十二、 結晶度分析 13
十三、 PHA聚合物機械性質分析 13
十四、 PHA聚合物分子量分析 14
第三章 結果 15
一、 嵌段PHA共聚物的生合成 15
二、 碳源種類對Cupriavidus sp. L7LΔwcaJ生長的關係 15
三、 利用電腦控制醱酵槽來大量合成PHA 16
四、 PHA嵌段共聚物熱性質的分析 17
五、 不同PHA嵌段共聚物機械性質之分析 (拉伸試驗) 18
六、 碳源濃度與PHA單體變化 18
七、 PHA分子量分析 18
第四章 討論 19
結論 22
參考文獻 23

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