本文研究光纖式光鑷夾系統，並用之捕捉酵母菌、人類膀胱癌細胞、人類慢性骨髓性白血病細胞和人類急性單核細胞白血病細胞，以進行分析研究。首先探討成本低之紅光雷射作為光鑷夾系統光源之可行性。由實驗數據顯示，紅光光鑷夾系統可以捕捉酵母菌及癌細胞等微小活體。一般的研究需要大量實驗樣本，使結果具備統計上的意義。但本研究所述光捕捉方法可以將細胞內部的生物化學組成變化即時且準確地反映在光學上的逃脫速度，故不需要大量實驗樣本。因此，本光捕捉方法具有低誤判率高鑑別率等特性。本研究有優化光鑷夾之流程，可降低光鑷夾對癌細胞之光損害。本研究結果顯示本光捕捉方法可達到鑑別酵母菌及癌細胞種類之功能。

In this study, we have successfully trapped yeasts, human bladder cancer cells, human chronic granulocytic leukemia cells and human acute myeloblastic leukemia cells by the optical tweezer system. We have implemented a low-cost optical tweezer system with a red laser. Usually, biomedical research requires a large number of experimental data and samples to obtain statistical results. However, the optical trapping method described in this study can accurately reflect the biochemical composition inside cells in terms of escape velocities, so a large number of experimental samples and data are not required. The present optical trapping method is proven to be able to differentiate various yeast types and cancel cell types. In addition, the fabrication process of the fiber microlens of the optical tweezer has been optimized. The optimization of trapping power can reduce the optical damage on the cells. The results show that the optical trapping method can identify yeast types and cancer cell types.

目錄
中文摘要 I
Abstract II
誌謝 III
目錄 V
圖目錄 VII
表目錄 XII
第1章 光鑷夾緒論 1
1.1 光鑷夾重要發展史 1
1.2 光鑷夾生醫應用 5
1.3 本實驗室光鑷夾研究 21
1.4 癌症常用檢驗法 37
1.5 研究動機 38
1.6 論文架構 39
第2章 光鑷夾研究方法 40
2.1 研究架構 40
2.2 光纖微透鏡製程 43
2.3 光鑷夾系統組裝 47
2.4 光捕捉實驗系統參數與測量 56
2.4.1 工作距離計算 61
2.4.2 逃脫速度量測 62
2.4.3 捕捉力與捕捉效率測定 63
2.4.4 癌細胞逃脫速度範圍 64
第3章 第三章結果與討論 65
3.1 光捕捉酵母菌 65
3.1.1 光捕捉酵母菌A之結果 66
3.1.2 光捕捉酵母菌B之結果 67
3.1.3 光捕捉酵母菌C之結果 68
3.1.4 分析與討論 69
3.2 光捕捉人類第二期膀胱癌細胞 71
3.2.1 系統捕捉功率10 mW之結果 72
3.2.2 系統捕捉功率15 mW之結果 73
3.2.3 系統捕捉功率20 mW之結果 73
3.2.4 分析與討論 74
3.3 光捕捉人類慢性骨髓性白血病細胞 75
3.3.1 系統捕捉功率10 mW之結果 77
3.3.2 系統捕捉功率15 mW之結果 77
3.3.3 系統捕捉功率20 mW之結果 78
3.3.4 分析與討論 78
3.4 光捕捉人類急性單核細胞白血病細胞 79
3.4.1 系統捕捉功率5 mW之結果 81
3.4.2 系統捕捉功率10 mW之結果 82
3.4.3 系統捕捉功率15 mW之結果 83
3.4.4 系統捕捉功率20 mW之結果 84
3.4.5 分析與討論 85
第4章 結論與未來工作 88
參考文獻 89
簡歷表 93

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