臺灣博碩士論文加值系統

蘆筍(Asparagus)是百合科植物 ，以宿根形式生長多年，來自北非、西亞。本研究以超音波萃取法取代傳統熱回流方法萃取常被視為農業廢棄物的蘆筍老莖及擬葉，以L9直交試驗的三個萃取變因(萃取時間45、50、55分鐘、萃取溫度40℃、50℃、60℃、固液比1:10、1:20、1:30)，來探討蘆筍老莖及擬葉萃取物在抗氧化活性及抑制黃嘌呤氧化酶活性最佳的萃取條件。抗氧化試驗的結果顯示， B1:10 組(萃取50分鐘，50℃，固液比1:10 )，在粗萃物濃度為0.5 mg/ml，清除DPPH自由基能力81.3 %，還原力試驗中以B1:20組(萃取50分鐘， 60℃，固液比1:20) 66.55 %的活性為佳，總抗氧化能力試驗則以B1:30組(萃取50分鐘，40℃，固液比1:30) 其活性70.57 %為最高，當粗萃物濃度5 mg/ml時螯合亞鐵能力89.42 %，粗萃物之總黃酮與總酚含量以B1:30組最多，分別為400 mg of Quercetin/g與89.91 mg of GAE/g。
黃嘌呤氧化酶抑制活性的試驗在粗萃物樣品濃度0.7 mg/ml時，抑制率前三名分別為B1:10組的51.96 ± 1.27 %，A1:10組(萃取40分鐘， 40℃，固液比1:10)的50.11 ± 7.87 %，B1:30組的49.02 ± 12.40 %。綜合蘆筍老莖及擬葉萃取物抗氧化活性與黃嘌呤氧化酶抑制活性的直交試驗結果，可以發現萃取時間50分鐘、溫度40~50℃、固液比1:10最佳萃取條件。

Asparagus (Asparagus) is a plant in the Liliaceae family that has grown for many years in the form of perennial roots and comes from North Africa and West Asia. In this study, the ultrasonic extraction method was used to replace the traditional heat reflux method to extract old asparagus stems and pseudo leaves, which are often regarded as agricultural waste, and the three extraction factors of the L9 orthogonal test (extraction time 45, 50, 55 minutes, extraction temperature 40℃, 50℃, 60℃, solid-to-liquid ratio 1:10, 1:20, 1:30), to explore the best extraction of asparagus old stem and pseudo-leaf extract in antioxidant activity and inhibition of xanthine oxidase activity condition. The results of the antioxidant test showed that the B1:10 group (extraction for 50 minutes, 50°C, solid-liquid ratio 1:10), at a crude extract concentration of 0.5 mg/ml, the ability to scavenge DPPH free radicals was 81.3%, and in the reducing power test The B1:20 group (extraction for 50 minutes, 60°C, solid-liquid ratio 1:20) is the best activity of 66.55 %, and the total antioxidant capacity test is based on the B1:30 group (extraction 50 minutes, 40°C, solid-liquid ratio 1 :30) Its activity is the highest at 70.57%. When the concentration of the crude extract is 5 mg/ml, the chelating capacity of ferrous iron is 89.42%. The content of total flavonoids and total phenols of the crude extract is the highest in the B1:30 group, respectively 400 mg of Quercetin/g and 89.91 mg of GAE/g. In the test of xanthine oxidase inhibitory activity, when the crude extract sample concentration was 0.7 mg/ml, the top three inhibitory rates were 51.96 ± 1.27% of the B1:10 group, and the A1:10 group (extraction for 40 minutes, 40°C, solid Liquid ratio 1:10) is 50.11 ± 7.87 %, B1:30 group is 49.02 ± 12.40 %. Based on the results of the orthogonal test of the antioxidant activity of the old asparagus stem and pseudo-leaf extract and the inhibitory activity of xanthine oxidase, it can be found that the extraction time is 50 minutes, the temperature is 40~50℃, and the solid-liquid ratio 1:10 is the best extraction condition.

摘要..........i
Abstract.........ii
誌謝..........iii
目錄..........iv
表目錄..........vi
圖目錄..........viii
第一章前言..........1
第二章文獻回顧..........2
2.1蘆筍介紹..........2
2.1.1蘆筍在台灣使用與栽培分布..........2
2.1.2蘆筍各部位之介紹..........3
蘆筍嫩莖(地上莖)..........3
2.1.3蘆筍整株製作天然草藥的功能..........4
2.1.4蘆筍(百合科)含有生理活性成分..........4
2.2高尿酸血症..........5
2.2.1黃嘌呤氧化酶..........5
2.2.2別嘌呤..........5
2.3超音波萃取法介紹..........6
2.3.1直交實驗運用與介紹..........7
2.3.2直交表設計..........7
2.3.4條件分析..........7
2.5抗氧化介紹..........8
2.5.1還原力測定原理..........8
2.5.2總抗氧化..........8
2.5.3黃酮類化合物..........9
2.5.4總酚化合物..........9
2.7研究目的..........10
2.8實驗架構..........11
第三章 材料與方法..........12
3.2.1自由基能力測定..........13
3.2.4螯合亞鐵子能力測定..........13
3.2.5還原能力..........14
3.2.2總抗氧化..........14
3.2.3總酚類含量測定..........15
3.2.4總黃酮含量測定..........15
3.2.5抑制黃嘌呤氧化酶活性能力..........16
第四章結果與討論..........19
4.1.1樣品萃取回收結果..........19
4.1.2極差分析結果..........19
4.1.3蘆筍擬葉粗萃..........19
4.1.4 蘆筍擬葉萃取物清除自由基DPPH之能力..........22
4.1.5蘆筍擬葉及母莖萃取物之檢測螯合亞鐵能力..........28
4.1.6蘆筍擬葉與母莖萃取物之還原能力..........34
4.1.7蘆筍擬葉與母莖萃取物之總抗氧化能力..........40
4.1.8蘆筍擬葉及母莖萃取物之總黃酮..........46
4.1.9蘆筍擬葉及母莖萃取物之總酚..........49
4.2.0蘆筍擬葉粗萃物抑制黃嘌呤氧化酶活性..........52
第五章總結..........56
參考資料..........58
附錄一蘆筍整體介紹..........61
附錄二蘆筍花、擬葉、果實介紹..........62
附錄三蘆筍擬葉枝..........63
附錄四蘆嫩莖..........64
附錄五蘆筍種植..........65
References..........72



 

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