本研究利用氨氮作為基質，並添加厭氧產氫微生物菌群HMB(Hydrogen-producing Microbiome)提供菌相來源馴養藻菌共生系統。實驗分為兩階段，第一階段以7個氨氮濃度進行批次實驗分別探討氨氮對光反應及暗反應單元之影響及最佳起始馴養濃度。第二階段為連續式藻柱實驗，分為光反應及暗反應兩個單元，以第一階段所得知之最佳啟動濃度馴養藻菌共生系統，並隨時間增加氨氮濃度，探討氨氮對於藻菌共生系統的影響，並以葉綠素a及總菌落數探討分光光度計波長600 nm以及640 nm可得知菌與藻的濃度之可行性。
第一階段實驗結果顯示，以50 mg/L NH4+-N啟動藻菌共生系統最佳，光反應氨氮去除率可達99%，暗反應則達76%，且藻類濃度有明顯上升之趨勢，其餘濃度因藻類濃度不足，導致氨氮濃度過高而抑制生長，雖然去除率達75%，但藻類濃度隨著時間減少，故第二階段之實驗以氨氮濃度50 mg/L NH4+-N啟動馴養，經馴養後依序增加氨氮濃度進入系統處理，氨氮去除率皆達75%以上，並以反應動力學計算出此系統之最大體積負荷為0.3 mg NH4+-N/MLVSS day。以葉綠素a及總菌落數與分光光度計之數據進行比較，結果得知總菌落數與波長600 nm所測出之生長趨勢不同，使用波長600 nm無法得知此系統菌之濃度；葉綠素a與波長640nm所測出之成長趨勢不同，故波長640 nm以得知系統中藻的濃度，其原因為相較於系統中的藻類，菌在此系統中屬於含量較少之部分，故無法以分光光度計測得菌之濃度，反之系統中大多為藻類則可以此檢測方式測得藻之濃度。

In this study, ammonia nitrogen was used as a substrate, and a hydrogen-producing microbiome(HMB) was added to provide a bacteriological source-derived algae-bacteria symbiosis system. The experiment was divided into two stages.In the first stage, batch experiments were carried out with 7 ammonia nitrogen concentrations to investigate the effects of ammonia nitrogen on photoreaction and dark reaction units and the optimal initial domestication concentration.The second stage is a continuous algae column experiment, which is divided into two units of photoreaction and dark reaction. The algae-bacteria symbiosis system is domesticated at the optimal starting concentration known in the first stage, and the ammonia nitrogen concentration is increased with time to explore the symbiotic relationship between ammonia and nitrogen. The influence of the system, and the chlorophyll a and the total number of colonies, the spectrophotometer wavelengths of 600 nm and 640 nm can be used to understand the feasibility of the concentration of bacteria and algae.
The results of the first stage experiment showed that the symbiotic system of algae was the best with 50 mg/L NH4+-N, the removal rate of photoreaction ammonia nitrogen was 99%, the dark reaction was 76%, and the algae concentration increased obviously. The concentration is low due to insufficient algae concentration, which leads to excessive ammonia concentration and inhibits growth. Although the removal rate is 75%, the algae concentration decreases with time. Therefore, the second stage experiment is started with ammonia nitrogen concentration of 50 mg/L NH4+-N. After domestication, the ammonia nitrogen concentration was increased in sequence and the ammonia nitrogen removal rate was over 75%. The maximum volumetric load of the system was calculated to be 0.3 mg NH4+-N/MLVSS day. The chlorophyll a and the total number of colonies were compared with the data of the spectrophotometer. The results showed that the total number of colonies was different from the growth trend measured at a wavelength of 600 nm. The concentration of the system was not known at a wavelength of 600 nm; chlorophyll a and the growth trend measured at a wavelength of 640 nm is different, so the wavelength of 640 nm is known to the concentration of algae in the system. The reason is that the bacteria are less in the system than the algae in the system, so it is impossible to split the light. The photometer measures the concentration of the bacteria, whereas the majority of the system is algae, the concentration of algae can be measured by this method.

摘要 i
Abstract ii
誌謝 iii
圖目錄 vi
表目錄 vii
第一章 前言 1
1.1研究背景 1
1.2研究目的 2
第二章 文獻回顧 3
2.1 氨氮廢水來源及危害 3
2.1.1 生活污水 4
2.1.2 石化業廢水 4
2.1.3 農牧廢水 5
2.2 藻 6
2.2.1 藻的種類 6
2.2.2 藻類生長因子 7
2.2.3 光合作用 9
2.2.4 藻類生長週期 11
2.3 菌 13
2.4 藻菌共生之概念及應用 14
2.5 氮循環 16
2.5.1 硝化作用 19
2.5.2 脫硝作用 21
第三章 材料與方法 22
3.1 實驗架構 22
3.2 實驗材料 24
3.2.1 植種材料 24
3.2.2 基質 25
3.2.3 營養鹽 26
3.2.4 微生物製劑 27
3.2.5 光合生物反應器 28
3.3 實驗方法與步驟 29
3.3.1 批次實驗 29
3.3.2 連續實驗 29
3.4 器材與設備 31
3.5 水質分析方法 34
3.5.1 水中氫離子濃度 34
3.5.2 藻菌生長量測定 35
3.5.3 鹼度 35
3.5.4 葉綠素a 37
3.5.5 總菌落 39
3.5.6 氨氮檢測 42
3.5.7 硝酸鹽氮檢測 43
3.5.8 總有機碳檢測 44
3.6 數據分析方法 45
第四章 結果與討論 46
4.1不同氨氮濃度對藻菌共生光反應與暗反應單元的影響 46
4.1.1 光反應組 46
4.1.2 暗反應組 50
4.1.3 光反應與暗反應反應速率之變化 54
4.1.4 小結 59
4.2 連續式藻菌共生系統之實驗 61
4.2.1酸鹼值之變化 61
4.2.2 鹼度之變化 63
4.2.3 MLVSS之變化 65
4.2.4 生長量之變化 67
4.2.5 氨氮、硝酸鹽氮之變化 72
4.2.6 總有機碳之變化 76
4.2.7 小結 77
第五章 結論與建議 79
5.1 結論 79
5.2 建議 80
第六章 參考文獻 81

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