紅黴素(Erythromycin, EM)是大環內脂類抗生素，廣泛應用於畜牧業。由於長期的濫用，對環境具有潛在的威脅。因此，設計出一種能監測微量紅黴素的便攜式感測器，對於環境安全至關重要。本研究結合光子晶體的製作並結合分子印記技術，製備出一種用於檢測紅黴素的二維分子印記的反蛋白石水凝膠感測器。以紅黴素做為目標模板，在聚合過程中形成具有特定標記物的水凝膠感測器。去除目標模板後，即可獲得與紅黴素分子互補的奈米孔穴水凝膠。藉由紅黴素與反蛋白石水凝膠孔洞上的特定分子孔穴相匹配的作用會使水凝膠體積改變。當雷射光照射在滴入不同濃度的紅黴素溶液的反蛋白石水凝膠後會在其背面產生繞射環直徑的變化，可快速偵測出微量紅黴素的濃度變化。當紅黴素的濃度從10-10 M增加到10-3 M時，繞射環的直徑會從4.0 cm增加到6.5 cm。這意味著隨著紅黴素濃度的增加，水凝膠中反蛋白石的孔隙間距會緊縮。此外，本研究所製備的感測器具有優異的專一性，無需使用貴重儀器即可對紅黴素進行快速偵檢，且有望在未來能應用在食品、土壤及水資源的檢測上。

Erythromycin (EM) is a macrolide antibiotic widely used in animal husbandry. Potential threat to the environment due to long-term abuse. Therefore, designing a portable sensor capable of monitoring trace amounts of erythromycin is crucial for environmental safety. In this study, combined with the fabrication of photonic crystals and molecular imprinting technology, a two-dimensional molecularly imprinted inverse opal hydrogel sensor was prepared for the detection of erythromycin. Using erythromycin as the target template, a hydrogel sensor with specific labels was formed during the polymerization process. After removing the target template, nanopore hydrogels complementary to erythromycin molecules can be obtained. The volume of the hydrogel is altered by the action of erythromycin that matches specific molecular cavities on the pores of the inverse opal hydrogel. When the laser light is irradiated on the inverse opal hydrogel dripped with different concentrations of erythromycin solution, the diameter of the diffraction ring will change on the back of the inverse opal hydrogel, and the concentration change of trace erythromycin can be quickly detected. When the concentration of erythromycin was increased from 10-10 M to 10-3 M, the diameter of the diffraction ring increased from 4.0 cm to 6.5 cm. This means that as the erythromycin concentration increases, the pore spacing of the inverse opal in the hydrogel tightens. In addition, the sensor prepared in this study has excellent specificity and can rapidly detect erythromycin without using expensive instruments, and is expected to be applied to the detection of food, soil and water resources in the future.

中文摘要………………………………………………………………………………i
英文摘要……………………………………………………………………………....ii
誌謝………...………………………………………………...……………………….iii
目錄………...………………………………………………………………………....iv
表目錄………...……………………………………………………………………….v
圖目錄………...……………………………………………………………………....vi
第一章 緒論………...……………………………………………………………….1
1.1 前言………...…………………………………………………………………..1
1.2 專利檢索…...…………………………………………………………………..3
第二章 實驗步驟與分析……………………………...………………………….....5
2.1 實驗步驟……………………………...…………………………......................5
2.1.1 聚苯乙烯奈米球合成……………………………………………..............5
2.1.2 單層聚苯乙烯奈米球陣列模板的製備…………………………..............5
2.1.3 高分子預聚合溶液的製備………………………………………..............5
2.1.4 紅黴素反蛋白石水凝膠的製備…………………………………..............6
2.2 實驗分析…………………………………………….........................................6
第三章 結果與討論………………………………………........................................7
3.1 蛋白石與反蛋白石的結構分析………………………………………….........7
3.1.1 聚苯乙烯奈米球粒徑分析…………………………………………..........7
3.1.2 單層聚苯乙烯蛋白石陣列結構分析…………………………………......7
3.1.3 聚苯乙烯反蛋白石水凝膠結構分析…………………………………......7
3.2 生物感測器分析……………………………………………………...……......7
3.2.1 水凝膠的繞射分析……………………………………………………......7
3.2.2 水凝膠對紅黴素微量濃度的色度響應………………………………......8
3.2.3 水凝膠的響應時間與再現性…………………………………………......8
3.2.4 水凝膠的專一性………………………………………………………......8
第四章 結論…………………………………………………………………..........10
第五章 未來展望……………………………...…………………………………...11
參考文獻 …………………………………………………………......................23

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