本論文應用免標記的導模共振感測器來檢測樣本溶液中Interleukin10 (IL-10) (亦稱為介白素-10)，IL-10在免疫系統中扮演著重要的調節角色。它具有抗發炎和免疫調節的功能。我們先以ELISA去測試，酵素結合免疫吸附分析法，Enzyme-linked immunosorbent assay （ELISA）是一種常用於定量檢測特定蛋白質或其他生物分子存在的方法。其基本原理是利用抗體與目標分子相互結合，然後通過酵素對染料的催化作用來測定目標分子的存在量。由於ELISA分析法僅需少量樣品（約100微升），及此具有高度靈敏度，能夠同時檢測多種抗體。因此，它被廣泛應用於分子生物學、生物化學和醫學研究等領域中。其靈敏度落在1600~12.5 pg/ml左右，但ELISA仍有檢驗時間長、成本較高的問題，因此我們利用導模共振感測器來做檢測，此光學感測技術在分析上實驗時間較短且成本較低，在實驗中，我們最初以PBS緩衝溶液作為基準線，反射光的初始數值為120 uW，加入一抗後，上升到167.9-170.5 uW之間，我們在以 PBS 清洗抗體，數值下降為139-140 uW之間，之後我們加入BSA，做為孔隙填補的材料，加入後數值上升為178-180 uW之間，再以PBS做清洗，此時數值掉到170-173 uW之間，之後再加入2*10-9g /ml抗原，此時數值緩緩上升到175-178 uW之間，再以PBS清洗，此時數值緩緩下跌至176.3uW，因為此數值大於加入抗原前的值170-173 uW，因此可以確定導模共振感測器表面有抗原，證實可測得 2*10-9g/ml的IL-10。

This paper uses a label-free guided-mode resonance (GMR) sensor to detect Interleukin 10 (IL-10) in sample solutions. IL-10 plays an essential regulatory role in the immune system, with anti-inflammatory and immunomodulatory functions. Initially, Enzyme-linked immunosorbent assay (ELISA) was used to test IL-10. ELISA is a commonly used method to quantitatively detect specific proteins or other biomolecules, using antibodies that bind to target molecules and an enzyme that catalyzes a dye to measure the presence of the target. ELISA requires only a small sample (about 100 microliters) and has high sensitivity, allowing simultaneous detection of multiple antibodies. It is widely used in molecular biology, biochemistry, and medical research, with a sensitivity range of 1600~12.5 pg/ml. However, ELISA has drawbacks, such as long testing times and high costs. Therefore, we utilized a GMR sensor for detection, which offers shorter analysis times and lower costs. In the experiment, we used a PBS buffer as a baseline, with the initial reflected light value at 120 uW. After adding the primary antibody, the value increased to between 167.9~170.5 uW. After washing the antibody with PBS, the value decreased to between 139-140 uW. Then, BSA was added as a blocking material, raising the value to 178-180 uW, followed by another PBS wash, which lowered the value to 170~173 uW. Finally, after adding 2 *10-9 g /ml of antigen, the value gradually increased to 175~178 uW, and another PBS wash decreased it to 176.3 uW. Since this value is greater than the pre-antigen addition value of 170~173 uW, it confirms the presence of antigen on the GMR sensor surface, demonstrating the ability to detect 2*10-9 g/ml of IL-10.

摘要........................... i
Abstract....................... ii
誌謝........................... iii
目錄........................... iv
表目錄......................... vi
圖目錄......................... vii
第一章 緒論..................... 1
1.1 研究目的與動機.............. 1
1.2 文獻回顧.................... 2
1.3 研究方法.................... 8
1.4 論文架構.................... 9
第二章 導模共振原理.............. 10
2.1 導模共振效應................ 10
2.2 嚴格耦合波分析法........ 10
第三章 GMR感測器種類............ 12
3.1 GMR感測器.................. 12
3.2穿透式光強度導模共振......... 14
第四章GMR元件製作與模擬......... 15
4.1 模擬軟體................... 15
4.2 GMR元件製程................ 17
4.2.1 PDMS微流道製作........... 17
4.2.2 TiO2 光柵製作........... 18
4.3 GMR元件材料................ 19
4.3.1 聚二甲基矽氧烷（PDMS)..... 19
4.3.2 二氧化鈦(TiO2)............ 20
第五章反射式強度量測系統......... 21
5.1 反射式強度量測系統光路結構... 21
5.1.1 精密旋轉平台規格.......... 21
5.1.2 3D列印所製成的機構及其功能. 22
5.1.3 懸臂歸零校正曲線.......... 25
5.2 軟體選用與程式使用........... 25
5.2.1 光功率計.................. 25
5.2.2 控制程式.................. 26
第六章IL-10檢測實驗與結果........ 27
6.1共振角量測................... 27
6.2 IL-10檢測實驗方法........... 29
6.2.1 ELISA與GMR分析法流程...... 29
6.2.2 EDC/NHS反應.............. 29
6.2.3 GMR分析方法............... 30
6.2.4 儀器及藥品來源............ 31
6.3 ELISA測量結果............... 32
6.3.1分析結果................... 32
6.4 GMR分析量測結果............. 33
第七章結論與未來展望............. 36
參考文獻........................ 37
Extended Abstract.............. 39

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