臺灣博碩士論文加值系統

本研究應用全場外差干涉術在動態反應的量測上，並完成了葡萄糖感測試片在偵測葡萄糖濃度之二維反應趨勢。由於感測試片上佈植之葡萄糖氧化酵素(GOx)具有對葡萄糖的化學反應專一的特性，故可完全反映待測溶液中葡萄糖濃度，不受溶液中其他化學成分之干擾。此外，結合全場外差干涉相位量測技術，可獲得感測試片上GOx與葡萄糖反應之二維狀態，有利於GOx佈植於玻璃板上之特性分析。此外，本研究並探討GOx與待測溶液間pH值差異對量測反應時間及量測靈敏度的影響，以及GOx濃度對量測反應時間及量測靈敏度的關聯性。由實驗結果顯示，本研究之反應時間優於2.2秒且系統解析度可達39.286 mg/dL。

In this study, we demonstrated a full-field heterodyne interferometer to analyze two dimensional (2D) dynamic phase variation of the chemical reaction between the testing sample and the glucose sensor. Because of high selectivity of the glucose oxidase (GOx), we can ensure the phase variation coming from the reaction between GOx and glucose dissolved in the complicated testing sample. Based on the advantages of the full-field heterodyne interferometer, we can investigate the 2D reaction behavior of the GOx under different immobilized pH value and concentration. According to our results, the best response time is shorter than 2.2 seconds observed in this study. We also obtained the calibration curve with high linearity within the glucose concentration of 100 ~ 600 mg/dL. Finally, we can get the resolution of this method can be reached to 39.286 mg/dL.

書名頁 i
論文口試委員審定書 ii
授權書 iii
中文摘要 iv
英文摘要 v
誌謝 vi
目錄 vii
表目錄 ix
圖目錄 x
第一章 緒論 1
1.1 背景簡介 1
1.2 文獻回顧 1
1.3 研究動機 4
1.4 論文架構 5
第二章 全場外差干涉術 6
2.1 使用電光晶體調製外差光源 6
2.2 外差干涉術原理 8
2.3 全場外差干涉術的相位解析方法 9
2.4 實驗架構原理 11
第三章 實驗結果 16
3.1 葡萄糖溶液調配 16
3.2 葡萄糖氧化感測試片之製作 17
3.3 量測方法與資料分析流程 19
3.4 量測結果 25
3.4.1 各種葡萄糖溶液濃度量測結果 25
3.4.2 各種GOx酸鹼值的量測結果 33
3.4.3 各種GOx濃度的量測結果 38
第四章 討論 44
4.1 誤差分析 44
4.1.1 相機取樣誤差 44
4.1.2 偏極混合誤差 45
4.1.3 環境振動誤差 46
4.2 系統解析度分析 48
4.3 試片特性分析 52
4.3.1 試片的酸鹼值分析 52
4.3.2 試片的濃度分析 55
4.4 小結 58
第五章 結論與未來展望 59
5.1 結論 59
5.2 未來展望 60
參考文獻 xii

[1] Pedrotti, “Introduction to Optics,” 3rd ed., Chap.1, (Prentice Hall), 1-6 (2007).
[2] Ajoy Ghatak, “Optics,” 3th ed., Chap.1, (McGraw-Hill), 1.5-1.8 (2005).
[3] K. J. Gasvik, “Optical Metrology,” 3rd ed., Chap.3, (John Wiley & Sons), 38-63 (2002).
[4] 林宸生，雷射精密量測設備，http://140.134.32.129/eleme/mea/chap12.html
[5] G. L. Cote, “Noninvasive optical glucose sensing-an overview,” J. Clin. Eng. 22, 253-259 (1997).
[6] K. H. Chen, C. C. Hsu, and D. C. Su, “Interferometric optical sensor for measuring glucose concentration,” Appl. Opt. 42, 5774-5776 (2003).
[7] Y. L. Yeh, “Real-time measurement of glucose concentration and average refractive index using a laser interferometer,” Opt. Laser Eng. 46, 666-670 (2008).
[8] X. Wang, A. Zhang, M. Zhi, A. V. Sokolov, and G. R. Welch, “Glucose concentration measured by the hybrid coherent anti-Stokes Raman-scattering technique,” Phys. Rev. A 81, 013813-6 (2010).
[9] X. D. Wang, H. X. Chen, T. Y. Zhou, Z.J. Lin, J. B. Zeng, Z. X. Xie, X. Chen, K. Y. Wong, G. N. Chen, and X. R. Wang, “Optical colorimetric sensor strip for direct readout glucose measurement,” Biosens. Bioelectron. 24, 3702-3705 (2009).
[10] J. Tenhunen , H. Kopola, and R. Myllyla “Non-invasive glucose measurement based on selective near infrared absorption; requirements on instrumentation and spectral range,” Measurement 24, 173–177 (1998).
[11] K. Maruo, M. Tsurugi, J. Chin, T. Ota, H. Arimoto, Y. Yamada, M. Tamura, M. Ishii, and Y. Ozaki, “Noninvasive Blood Glucose Assay Using a Newly Developed Near-Infrared System,” IEEE Photonics Society 9, 322-330 (2003).
[12] Y. Xu, X. Liu, Y. Ding , L. Luo, Y. Wang, Y. Zhang, and Y. Xu “Preparation and electrochemical investigation of a nano-structured material Ni2+/MgFe layered double hydroxide as a glucose biosensor.” Appl. Clay Sci. 52, 322–327 (2011).
[13] B. Haghighi, S. Bozorgzadeha, and L. Gortonb “Fabrication of a novel electrochemiluminescence glucose biosensor using Au nanoparticles decorated multiwalled carbon nanotubes,” Sensor Actuat B-Chem 155, 577-583 (2011).
[14] G. Changa, Y. Tatsua, T. Gotob, H. Imaishi, and K. Morigaki “Glucose concentration determination based on silica sol–gel encapsulated glucose oxidase optical biosensor arrays,” Talanta 83, 61–65 (2010).
[15] O.O. Soldatkin, V. M. Peshkova, S. V. Dzyadevych, A. P. Soldatkin, N. Jaffrezic-Renault, and A. V. El''skaya “Novel sucrose three-enzyme conductometric biosensor,” Mater Sci. and Eng. C 28, 959–964 (2008).
[16] 廖志曄，「非侵入式葡萄糖濃度量測技術之研究」，國立台灣大學，碩士論文，民國92年。
[17] K. Takahara, M. Sasaki, Arthur J. Tomisek and S. Natelson “Sensitive o-toluidine reagent for glucose assay at moderately elevated temperatures,” Microchem J 19, 319-329 (1974).
[18] J. Kaiser, “Diabetes efforts to get $300 million,” Science 277, 755 (1997).
[19] 陳彥良，「顯微干涉術在折射率及表面形貌之量測應用」，國立交通大學，博士論文，民國99年。
[20] A. Yariv and P. Yeh, “Optical Wave in Crystals,” 1st ed., Chap.8, (John Wiley & Sons), 276-287 (1984).
[21] Y. L. Chen and D. C. Su, “A method for determining full-field absolute phase in the common-path heterodyne interferometer with an electro-optic modulator,” Appl. Opt. 47, 6518-6523 (2008).
[22] G. E. Sommargren, “Optical heterodyne profilometry,” Appl. Opt. 20, 610-618 (1981).
[23] D. C. Ghiglia and M. D. Pritt, “Two-dimensional phase unwrapping: theory, algorithms, and software,” Wiley, New York, (1998).
[24] IEEE, “Standard for terminology and test methods for analog to digital converters,” IEEE Std 1241-2000, 25-29 (2000).
[25] S. Reichelt and H. Zappe, “Combined Twyman-Green and Mach-Zehnder interferometer for microlens testing,” Appl. Opt. 44, 5786-5792 (2005).
[26] S. A. Yamannaka, F. Nishida, L. M. Ellerby, C. R. Nishida, B. Dunn, J. S. Valentine, and J. I. Zink, “Immobilization of glucose oxidase on glass with two methods,” Chem. Mater. 4, 495-497 (1992).
[27] Guideline on standard operation procedures for clinical chemistry: CSF glucose-glucose oxidase method, http://www.searo.who.int/EN/Section10/
Section17/Section53/Section481_1767.html
[28] R. Luo, H. Li, and K. Y. Lam, “Modeling the effect of environmental solution pH on the mechanical characteristics of glucose-sensitive hydrogels,” Biomaterials. 30, 690-700 (2009).
[29] H. J. Bright and M. Appleby, “The pH dependence of individual step in the glucose oxidase,” J. Biol. Chem. 244, 3625-3634 (1969).
[30] Z. C. Jian, Y. L. Chen, H. C. Hsieh, P. J. Hsieh, and D. C. Su, “Optimal condition for full-field heterodyne interferometry,” Opt. Eng. 46, 115604-6 (2007).
[31] M. H. Chiu, J. Y. Lee, and D. C. Su, “Complex refractive-index measurement base on Fresnel’s equations and uses of heterodyne interferometry,” Appl. Opt. 38, 4047-4053 (1999).
[32] C. M. Wu and R. D. Deslattes, “Analytical modeling of the periodic nonlinearity in heterdyne interferometry,” Appl. Opt. 37, 6696-6700 (1998).
[33] W. Hou and G. Wilkening, ”Investigation and compensation of the nonlinearity in heterdyne interferometers,” Prec. Eng. 14, 91-98 (1992).
[34] A. E. Rosenbluth and N. Bobroff, “Optical sources of nonlinearity in heterdyne interferometers,” Prec. Eng. 12, 7-11 (1990).
[35] 黃雲晟，「旋光外差干涉術於葡萄糖氧化酵素感測試片之研究」，元智大學，碩士論文，民國99年。