臺灣博碩士論文加值系統

牛樟芝(Antrodia cinnamomea)為臺灣傳統中藥，具有抗氧化及治療癌症的功效，其中包括抗血癌。血癌細胞為一群分化不完全的細胞，因此誘導血癌細胞分化，為一種急性血癌的主要治療策略，但是否牛樟芝具有促進血癌細胞分化的能力，目前並沒有相關的文獻報導。本研究主要為探討牛樟芝純化物誘導血癌細胞HL-60及KG-1分化的現象。在本實驗中，以五種不同的牛樟芝純化物(Antcin A, B, C, Zhankuic acid C及 Methyl antcinate A)，處理HL-60及KG-1細胞後，觀察其生長及分化之情形。其結果顯示，HL-60及KG-1細胞在牛樟芝純化物，Antcin B及Zhankuic acid C，處理後，產生細胞數減少及存活率降低之現象，但在另外三種牛樟芝純化物(Antcin A, C, 及 Methyl antcinate A)中，並未看到明顯之現象。此外，兩株細胞皆可發現細胞分化之型態變化。利用流式細胞儀分析出單核球分化之細胞表面特異性分子CD14及CD11b表現量會增加。由實驗結果證實， Antcin A, B, C, Zhankuic acid C及 Methyl antcinate A可抑制血癌細胞HL-60及KG-1之生長及誘導其分化為單核球細胞。此結果顯示牛樟芝中所含的化學物質具有誘導血癌細胞分化及抑制血癌細胞生長的能力，而具有抗血癌之潛力。

Antrodia cinnamomea is a well known medicinal mushroom, and it has been shown to exhibit antioxidant and anticancer treatment effects. Cell differentiation is essential for normal growth and hematopoiesis. Inducing myelopoiesis can potentially serve as differentiation therapy for acute myeloid leukemia (AML). In this study, the ability of pure compounds purified from Antrodia cinnamomea to induce differentiation was studied in culture human myelocytic leukemia HL-60 and KG-1 cells. Treatment of HL-60 and KG-1 cells with Antcin B and Zhankuic acid C significanitly induced cell viability. In contrast, Antcin A, C, and Methyl antcinate A did not shown. The morphological investigation, and used the flow cytometry that also increased the levels of monocyte differentiation markers, CD14 and CD11b in both two cells. The data suggest that Antcin A, B, C, Zhankuic acid C and Methyl antcinate A exerts growth inhibition and monocyte-like differentiation on HL-60 and KG-1 cells through induction differentiation and that chemical compounds purified from Antrodia cinnamomea may have anticancer properties valuable for application in drug products.

目錄…………………………………………………………………………i
圖表目錄………………………………………………………………..….ii
中文摘要…………………………………………………………….…….iv
英文摘要……………………………………………………….………….vi
一、 前言……………………………………………………..………….1
二、 研究動機………………………………………………………….23
三、 研究策略……………………………………...…………………..25
四、 實驗設計架構…………………………………………………….26
五、 實驗材料與方法………………………………………………….27
六、 實驗結果………………………………………………….………33
七、 討論……………………………………………………...………..36
八、 結論與未來展望…………………………………………………43
九、 圖表……………………………………………………….……44
十、 參考文獻………………………………………………...……..…63

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