樟芝（Antrodia cinnamomea）為台灣特有的藥用菇類，但由於樟芝生長緩慢且取之不易，因此近年來大多使用人工培養，本研究探討以不同品種香蕉果實作為樟芝培養基質之複合碳源，探討碳源改變對樟芝多醣體合成與脂肪酶活性之影響。試驗結果顯示，香蕉果實作為培養基碳源是可行的；北蕉未催熟碳源全取代有最佳之多醣體產量及脂肪酶活性，其多醣體為3.5770 gmL-1及脂肪酶活性5.613 UmL-1，可利用此特性將香蕉果實作為替代培養基碳源，替代傳統化學培養基，做為香蕉產銷失衡時之對應方案。

Antrodia cinnamomea is a kind of precious medicinal mushrooms in Taiwan, But due Antrodia cinnamomea very slow grownd, so in recent years, mostly using artificial cultivationto, this study explores the matrix of the composite carbon as Antrodia cinnamomea cultured with different varieties of banana fruits ources to explore the carbon source to change the effect of Antrodia cinnamomea polysaccharides synthesis and lipase activity. The results show that the banana fruit as a medium carbon source is feasible; North banana ripening is not carbon source to replace the whole polysaccharide production and lipase activity, polysaccharides 3.5770 gmL-1 and lipase activity of 5.613 UmL-1, this feature can be used banana fruit as an alternative medium carbon source, an alternative to traditional chemical medium, as the imbalance in banana production and marketing of the corresponding program.

摘要
Abstract:
誌謝
目錄
第一章 緒論
1.1 研究背景
1.2 研究背景
1.3 研究目的
第二章 文獻回顧
2.1 食藥用菇類簡介
2.2 食藥用菇類的生理活性
2.3 食用藥菇液態培養之特性
2.4 人工培養基的組成
2.4.1 碳源
2.4.2氮源
2.4.3 碳氮比
2.4.4 無機鹽類
2.5 人工培養環境因子
2.5.1 搖晃速率與剪切力
2.5.2 溫度
2.5.3 pH值
2.6 食用藥菇液態培養之特性
2.7香蕉
2.8樟芝相關研究
2.9脂肪酶相關研究
2.10醣類與脂質在微生物學及生物化學基礎理論
第三章 材料與方法
3.1 供試材料
3.1.1 樟芝來源
3. 1.2 樟芝培養基複合碳源之來源
3.2 處理方法
3.2.1 樟芝繼代與保存之固態培養基(MEA)
3.2.2 樟芝菌絲體之保存
3.2.3 樟芝菌絲體活化與繼代培養
3. 2.4 香蕉果實預處理
3.2.5 酵母抽取物液態培養基(Yeast Extract, YM)與香蕉果實複合碳源培養基
3.2.6 樟芝液態培養醱酵液處理
3.3 檢測項目與方法
3.3.1 樟芝培養液之多醣萃取與分析
3.3.2 樟芝醱酵液之脂肪酶活性測定
3.4 研究數據統計分析 SAS統計分析軟體分析
3.5 實驗流程圖
3.6 試驗藥品與儀器
3.6.1 實驗藥品
表3-4. 實驗藥品成分與廠牌
3.6. 2 實驗儀器
表3-5. 實驗儀器設備之廠商與規格
第4章 結果與討論
4.1結果探討
4.1.1北蕉及南華蕉果實作為樟芝培養基對合成多醣體及脂肪酶活性影響
4.1.2北蕉及南華蕉果實催熟及未催熟作為樟芝培養基對合成多醣體及脂肪酶活性影響
4.1.3 北蕉及南華蕉果實催熟及未催熟調整添加比例作為樟芝培養基對合成多醣體及脂肪酶活性影響
4.1.4 添加不同品種香蕉複合碳源培養基對樟芝菌絲體之影響
第5章 結論
參考文獻
附錄

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