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Chitin was known to be a wound healing enhancer, and
membrane made from crab shell has been commercialized as a
dressing for skin defect. Since fungi with chitin component in
their cell wall, it''s a charming idea to use fungal mycelia as a
material for wound management. We used a new membrane, which was
made from extracted waste of Ganoderma tsugae manufacturing, to
evaluate the potential as a wound dressing. The residue of
fruiting body of G. tsugae previously extracted with hot water
was further trted with 95% EtOH, 0.1N HCl, 1N NaOH at 85℃, and
0.1% sodium hypochlorate to remove undesirable component,
especially the protein and pigment. The final product was white
powder, about half of the original material by weight, with
filamentous structure in mycelial form under scanning electronic
microscopy. Chemical analysis revealed that it was a copolymer
of glucose ( 60% ) and N-acetyl-glucosamine ( 40% ). The white
powder was then woven into thin, porous circular sheet of 7.0 cm
in diameter and 0.1 ~ 0.mm in thickness by filtration and
lyophylization. Female guinea pigs weighing 380 ~ 480 g were
used. After anesthetized with ketamine and pentobarbital by
intraperitoneal injection, dorsal skin hairs were removed with
electric clipper. Two wounds as mirror-image were made on the
back by dissecting 1.5 x 1.5 cm2 skin in full thickness.
Ganoderma membrane was applied
on one wound randomly and gauze or commercial chitin membrane
was u sed on the other. The wound areas were measured and
photographed at days 5, 10 , 15, 20 postoperation.
Histological examinations were also performed to revea l the
interaction of tissue and dressing. The Ganoderma wound areas
were signi ficant smaller than gauze''s on day 10. There was no
significant difference of wound size between chitin sheet
from crab and Ganoderma. In the second part of the present
investing, fibrobsts from dermis layer of guinea pigs were used
t o examined the effect of proliferation and migration
enhanced by Ganoderma res idue. Cell numbers of fibroblast
culture in DMEM were counted on the days 1 , 3 , 6 , 9 and 13
with or without suspending dressing materials (0.01 w/v). Mig
ration of fibroblast were measured by removing a part of the
grown cells on DM EM plate and counting the cells migrated
aross the margin of the cut for 5 day s. In addition, a small
hole (1 mm in diameter) was punched at the center aga rose
(1%) plate suspended with dressing materials and 1-1.5×103
fibroblasts w ere put into the hole, then DMEM was flooded
over agarose surface. Microscopic observation lasted for 5
days on the migration of fibroblast and the number c ell moved
out of the hole were counted. All the results indicated that the
Gan oderma suspension enhanced proliferation and migration of
fibroblast, signific antly.
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