臺灣博碩士論文加值系統

分子氧之供給策略，對於微生物發酵，非常重要。魯氏酵母菌，為工業應用之重要菌株。本研究探討溶氧動力學對發酵液溶氧之影響，間歇加壓供氧對細胞生長之影響，以及供氧方式對魯氏酵母攝取發酵液中三價鉻離子之影響。實驗結果顯示，壓力對溶氧之影響模式，符合亨利定律。發酵液中之葡萄糖影響飽和溶氧，與溶氧速率。間歇加壓供氧，因跨膜壓差之助，增加胞內溶氧，與氧利用效率，發酵之菌體產率高於連續供氧。供氧充足，魯氏酵母菌不產生乙醇，亦不攝取發酵液中之鉻離子，生產胞內GTF之供氧策略，與生產菌體不同。

Strategy of supplying molecular oxygen is very important in microbial fermentation. Zygosaccharomyces rouxii is a yeast strain of many applications in the biochemical industries. This research investigated the dissolving dynamics of molecular oxygen in aqueous fermentation medium, intermittent pressurized oxygenation for cell production, and the effect of oxygen supplement on the uptake of trivalent chromium in the medium by the cell. Experimental results revealed that Henry’s Law is applicable in the ranges of pressure and glucose concentration adopted in this study. Intermittent pressurized oxygenation facilitated the cross-membrane oxygen transfer and enhanced growth efficiency. Cr3+ uptake to synthesize intracellular GTF needs anaerobic environment. Experimental results revealed high efficiency of GTF in terms of glucose metabolism and growth rate.

中文摘要 I
Abstract II
目錄 III
表目錄 VI
圖目錄 VII
第一章 緒論 1
第二章 文獻回顧 2
第三章 實驗裝置與方法 15
3.1 本章摘要 15
3.2 溶氧動力學實驗裝置 15
3.3 間歇加壓供氧發酵 16
3.4 胞內 GTF 生產 17
第四章 溶氧動力學 22
4.1 本章摘要 22
4.2 研究背景 22
4.2.1 熱力學 23
4.2.2 溶氧動力學 27
4.3 材料與方法 30
4.4 結果與討論 30
4.4.1 溶氧時間曲線與亨利常數 30
4.4.2 溶氧反應級數 32
4.4.3 糖濃度之影響 33
4.4.4 壓力效應 34
4.5 結論 35
第五章 間歇加壓供氧發酵生產菌體 44
5.1 本章摘要 44
5.2 研究背景 44
5.3 材料與方法 47
5.3.1 菌株與操作 47
5.3.2 分析 47
5.3.3 混合時間量測 48
5.3.4 數學模式 49
5.4 結果與討論 50
5.4.1 溶氧速率 50
5.4.2 混合時間 52
5.4.3 發酵時間曲線 53
5.4.4 生長曲線與數學模式 54
5.4.5 間歇加壓供氧 55
5.4.6 生長動力學 56
5.4.7 供氧量 57
5.4.8 菌體生長效率 59
5.5 結論 59
第六章 魯氏酵母菌發酵生產GTF 70
6.1 本章摘要 70
6.2 研究背景 70
6.3 材料與方法 72
6.4 結果與討論 73
6.4.1生長曲線與鉻之攝取 74
6.4.2 葡萄糖與乙醇之時間曲線 76
6.4.3 供氧模式與動力學 77
6.5 結論 79
第七章 總結 86
參考文獻 88
發表文章 95

 
表 目 錄

表2-1 真菌分類 3
表5-1生長動力學參數之比較 61
表6-1供氧模式與動力學參數之比較 80

 
圖 目 錄

圖2-1 典型酵母菌細胞之構造 7
圖3-1 Lutron溶氧感測器 DO-5510 18
圖3-2 溶氧實驗2 L廣口血清瓶蓋設施圖 19
圖3-3 溶氧實驗裝置圖 20
圖3-4 間歇加壓供氧發酵裝置圖 21
圖4-1氧氣溶於水之化學平衡反應示意圖 24
圖4-2不同壓力之水中溶氧時間曲線之影響 37
圖4-3以 –Ln(C*-C) 對時間作圖 38
圖4-4 5%葡萄糖水溶夜中壓力對溶氧時間曲線之影響 39
圖4-5 10%葡萄糖水溶夜中壓力對溶氧時間曲線之影響 40
圖4-6 15%葡萄糖水溶夜中壓力對溶氧時間曲線之影響 41
圖4-7 2 psig葡萄糖水溶夜濃度對溶氧時間曲線之影響 42
圖4-8 5 psig葡萄糖水溶夜濃度對溶氧時間曲線之影響 43
圖 5-1 溶氧時間曲線比較 62
圖5-2 400 rpm攪拌混和時間實驗灰階代表染料相對濃度 63
圖5-3 混和時間與攪拌速率之關係圖 64
圖5-4 時間曲線，0 psig通氣0.44 vvm，洩壓閥開啟 65
圖5-5 時間曲線，通氣增壓至 2.5 psig，維持壓力2分鐘，開閥洩壓30秒 66
圖5-6 時間曲線，通氣增壓至 5 psig，維持壓力2分鐘，開閥洩壓30秒 67
圖5-7 時間曲線，通氣增壓至 10 psig，維持壓力2分鐘，開閥洩壓30秒 68
圖5-8 時間曲線，5 psig連續通氣0.44 vvm 69
圖6-1 三價鉻離子濃度之原子吸收光譜儀檢量線 81
圖6-2 2.5 psig-20min-30sec頂部間歇供氧之菌體生長與Cr3+濃度之時間曲線 82
圖6-3 2.5 psig-20min-30sec頂部間歇供氧模式葡萄糖與乙醇濃度之時間曲線 83
圖6-4 2.5 psig-20min-30sec起始 5 h 頂部間歇供氧之菌體生長與Cr3+濃度之時間曲線 84
圖6-5 2.5 psig-20min-30sec起始 5 h 頂部間歇供氧模式葡萄糖與乙醇濃度之時間曲線 85

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