|
In this study, the observation of growth traits and morphology of Antrodia camphorata on Cinnamomum kanehirai Hayata basswood and Petri dish (plate) were investigated. The optical microscope was also employed to examined the growth characteristics and eventually draw its life cycle. It was found that the germination of Antrodia camphorata basidiospore and growth seal tightness of Antrodia camphorata mycelium had a great impact on fruiting bodies. Increasing the humidity and sufficient air supply are crucial for fruiting bodies formation especially when mycelium appear in the notch, lateral, or bottom of basswood. The color of fruiting bodies of Antrodia camphorata in basswood was begin with light pink followed by red with mist white and then turns red to yellow during 11 months investigation. Meanwhile, Antrodia camphorata basidiospores was found in the basswood in the initial of cultivation, followed by hyphae (1st month), basidiomycota (2nd month), mycelial growth from basidiospores (derived from splitting of swelling basidiocarp) (3rd to 6th months), arthrospore (8th month), mycelium bonding (9th month), basidiocarp and basidiospores (10th month), and basidiocarp (11th month) was observed under microscope.
The growth of Antrodia camphorata in the plate was affected by nutrients. The Antrodia camphorata mycelium was inoculated at the center of the Petri dish and the white mycelium was observed at day 4. The red myceliums begin growth from white layer edge after 8th day. Subsequently, half of the plate spread with the white-hair-like red mycelium at 12 to 16 days of cultivation. The basidiospore was found in the inoculum at day 0 of culture under microscope. The growth of hyphae and clamp connections proceed for 2 to 4 days and changing to skeletal hyphae and arthrospore at 6th to 8th day. At 12th day, the arthrospore was fusion with expanded basidiocarp. The basidiocarp becomes mature and turns to basidiomycota after two weeks cultivation. Finally, the mature basidiomycota split to basidiospore at 16th day of inoculation. The life cycle of Antrodia camphorata is completed.
|