臺灣博碩士論文加值系統

本研究主要是研究分離與鑑定樟之深層醱酵液之化學成份。
將樟芝深層醱酵液凍乾粉於室溫下以甲醇浸泡三次，每次24小時。甲醇抽提物經減壓濃縮後再利用乙酸乙酯與水分配萃取，取乙酸乙酯層濃縮後以重力管柱層析法 (Column Chromatography, C. C.)粗分，接續利用薄層層析 (Thin Layer Chromatography, TLC)、快速管柱分析 (Flash Column Chromatography, F. C. C.)、高效能液相層析法 (High Performance Liquid Chromatography, HPLC)、再結晶等方法，分離純化，經光譜鑑定得到16個化合物，其中有4個為新化合物，2個為首分天然物。
這些化合物概分為2個Steroids、4個Aromatic、2個Furanoid、2個Lactone及其他類6個。 此16個化合物皆與文獻中從天然樟芝子實體所分離出之化合物結構不同。

Antrodia camphorata is a well-known fungus that is believed to protect liver function. The study was on isolation and identification of the chemical constituents of Antrodia camphorata submerged whole broth. Powder of Antrodia camphorata submerged whole broth was extracted with MeOH at room temperature three times (1d each time). The extract was concentrated in vacuo to yield a residue which was suspended in water, and this phase was partitioned with ethyl acetate three times. The combined ethyl acetate layers afforded a black syrup which was separated y open column chromatography, thin layer chromatography, flash column chromatography, high performance liquid chromatography and recrystallization to afford 16 compounds. Among these compounds, four are new compounds and two are the first isolated from natural materials. These compounds are classified to 2 sterols, 4 aromatics, 2 furanoids, 2 lactones and 6 others. These compounds are all different from those in the fruit bodies of Antrodia camphorata.

目 錄
頁次
表目錄......................................................Ⅴ
圖目錄......................................................Ⅵ
中文摘要....................................................Ⅰ
英文摘要....................................................Ⅱ
第一章 前言................................................1
第二章 文獻回顧............................................2
一、樟芝簡介.................................................2
(一)樟芝子實體...............................................2
(二)樟芝深層醱酵液...........................................3
(三)樟芝與靈芝之比較.........................................5
二、樟芝之生理及藥理活性.....................................7
(一)功能性試驗...............................................8
(二)安全性試驗..............................................14
三、樟芝之一般成份分析......................................15
四、樟芝成份分析及結構鑑定之相關研究........................18
(一)、樟芝子實體............................................24
(二)、樟芝深層醱酵液........................................27
第三章 結果與討論.........................................28
第一節 材料與方法.........................................28
一、材料....................................................28
二、儀器................................................... 28
三、藥品....................................................29
四、方法....................................................29
第二節 化合物之分析與鑑定.................................38
第四章 結論...............................................85
第五章 參考文獻...........................................89

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