本實驗以230 mg/kg 之Nicotinamide注射於大白鼠腹腔，經過15分鐘後再以120 mg/kg 之Streptozotocin注射於腹腔，以誘導大白鼠產生糖尿病。待一周之後，將實驗動物分成一組健康的對照組及三組帶糖尿病的實驗組，三組實驗組分別餵食(1).海洋深層水及一般飼料(2).逆滲透水及一般飼料搭配輔助基材(3).海洋深層水及一般飼料搭配輔助基材。持續餵食之後在短期(7天)、中期(21天)及長期(40天)時間點分別取出血液測量其血糖值及鈣離子濃度。
結果顯示：(1)組的結果顯示，一般飼料含有比山藥更多的鈣質，以其餵食下的糖尿病大白鼠，其血液之鈣離子濃度在40天之內始終維持穩定的現象，但是糖尿病的情況並未得到改善。相反的，(2)及(3)組的結果顯示，經過一週，大白鼠的血糖值下降，於第三週(20天)時血糖值甚至下降到與正常值幾乎一樣，表示山藥對於糖尿病患是一種好的飲食選擇。從每日測量大白鼠體重的增加量及飲水量的結果與(1)組之大白鼠相比較，發現(2)及(3)組的大白鼠體重的增加量較多而飲水量較少，所以亦可證明，山藥對於糖尿病病患而言，其症狀略有改善；不過(2)及(3)組的結果卻顯示，大白鼠血液之鈣離子濃度在20天左右下降，於40天時仍偏低，表示山藥並不能改善糖尿病病患鈣質流失的情況；除此之外，深層海水對於鈣質流失的情況僅略有幫助，要能達到充分的補充鈣質流失必須仰賴高鈣食品。

We used 230 mg/kg Nicotinamide and 120 mg/kg Streptozotocin to treat SD rats by intraperitoneal injection. After they became diabetic model animal, they were divided into four groups: three experimental group rats and one health control group. We fed three experimental
groups by (1) DOW and general food, (2) RO water and Chinese yam mix with general food, and (3) DOW and Chinese yam mix with general food, respectively. Their blood sugar and calcium concentrations in plasma were quantified after seven days, twenty days and forty days.
Results: The data from group (1) confirm that the general food contains more calcareous than Chinese yam, because the blood concentration of calcium ions within the 40 days remained stable. However, the high concentration of glucose in the blood was not improved. In contrast, the data from group (2) and group (3) reveal that blood glucose levels were reduced after a week. After 20 days, the blood glucose levels were nearly the same as the control group. Therefore, Chinese yam is a good food for diabetic patients. The rats in group (2) and group (3) exhibited an increase in body weight and decrease in the required volume of daily drinking water, compared to the rats in group (1). This result further indicated that the Chinese yam might help to Diabetic patients. However, the blood calcium ion concentration in group (2) and (3) reduced from 20 days and 40 days. Basically, the Chinese yam can not help osteoporosis but the DOW can improve the osteoporosis slightly. More calcareous source needs to be taken for diabetic patients.

中文摘要 i
Abstract iii
目錄 iv
第一章 緒論 1
1.1前言 1
第二章 文獻回顧 5
2.1海洋深層水 5
2.1.1海洋深層水的定義 5
2.1.2海洋深層水的優點 7
2.1.3台灣海洋深層水的發展狀況 9
2.1.4台灣海洋深層水的相關研究及應用 10
2.1.5其他國家海洋深層水的發展狀況 14
2.1.6海洋深層水藥理作用 22
2.2糖尿病 22
2.2.1糖尿病簡介 22
2.2.2糖尿病分類 23
2.2.3糖尿病的診斷標準 25
2.2.4糖尿病與迸發症 26
2.2.5糖尿病與骨質疏鬆症 28
2.3山藥 29
2.3.1山藥介紹 29
2.3.2山藥炮製方法 30
2.2.3山藥的藥理作用 31
2.2.4山藥對於糖尿病的藥理作用 31
第三章 實驗方法與實驗架構 33
3.1實驗架構 33
3.2實驗流程圖 34
3.3材料 35
3.4實驗方法 36
3.4.1大白鼠之飼養 36
3.4.2誘導產生高血糖動物模型 37
3.4.3分成實驗組及對照組 38
3.4.4測量各組大白鼠之體重變化及飲水量 38
3.4.5在預定時間點採取大白鼠血液，測定血糖濃度 39
3.4.6在預定時間點採取大白鼠血液，測定血漿中鈣離子濃度 40
3.4.7在各階段評估大白鼠糖尿病狀況 48
第四章 實驗結果 49
4.1.製作鈣離子濃度檢量線 49
4.2.建立高血糖動物模型 49
4.3鈣離子濃度測量結果 50
4.4血糖濃度測量結果 51
第五章 討論 61
參考文獻 63
附圖 70

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