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Purposes: We use a new Ganoderma membrane, which is made from extracted wasteof fruiting body derived from Ganoderma tsugae, to evaluate the effect of as awound dressing. Chemical analysis revealed the Ganoderma membrane is acopolymer of glucose(60%) and N-acetyl-D-glucosamine(40%). Animals: FemaleWistar rats weighing 300-400g were used. After anesthetized with ketamine andpentobarbital by intraperitoneal injection, dorsal skin hairs were removedwith electric clipper. Two wounds as mirror-image were made on the back bydissecting 2*2 cm2 skins in full thickness. Ganoderma membrane was applied onone wound randomly and gauze was used on the other. These animals were cagedindividually with neck collars, and fed regular rat chow and water ad libitum.The wound areas were measured on the 4th, 8th, 12th, 16th, 20th day afterthese procedures. Histological examinations of wounds were also performed onthe 4th, 7th, 16th day postoperatively. Implantation of these materials wasdone to reveal the interaction of tissue and dressing. These area data wereanalyzed with paired Student''s T test. Results: The Ganoderma wound areas weresignificant smaller than gauze''s. Histological observation shows Ganodermamembrane induces mild inflammation and attracts polymorphonuclear leukocytes'' aggregation around the margin. These are many macrophages and giant cellsinfiltrating gauze (foreign body reaction). Conclusion: The healing ofGanoderma wound is more smooth than gauze''s. It costs to perform further teststo evaluate the possible clinical application due to the enhanced woundhealing effect of Ganoderma membrane.
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