臺灣博碩士論文加值系統

長久以來，稻米一直是亞洲許多國家與台灣的主要糧食來源，亦為重要的經濟作物。稻米含有許多抗氧化與抗發炎的物質，主要存在糙米的糠層與胚芽中。流行病學研究指出，攝食較多含有抗氧化成分的食物與降低多種人類疾病的發病率與死亡率有關。所以，若以全穀類米食取代普遍較常食用的精白米，作為膳食中的主食來源，應具有促進健康之潛力。本研究探討攝食米食對於小鼠肝臟與大腸抗氧化能力之影響，實驗動物採用兩種不同品系的小鼠：近交系C57BL/6JNarl雄性小鼠（國家實驗動物中心）與遠交系ICR雄性小鼠（樂斯科生技公司）。依實驗飼料組成分成五組：AIN-93G組（93G）、高蛋白質AIN-93G組（modified AIN-93G, M93G）、Chow diet（Chow）組、高蛋白質白米組（polished rice, PR）與高蛋白質米糠加糙米組（brown rice enriched with rice bran, RB）。實驗期間給予各組小鼠配方飼料並定期記錄攝食量與體重，飼養四週後犧牲。分析項目：肝臟與近端大腸抗氧化酵素、大腸長度與遠端大腸組織免疫染色。抗氧化酵素包括榖胱甘肽過氧化酶、榖胱甘肽還原酶、榖胱甘肽硫基轉移酶、超氧歧化酶與過氧化氫酶；組織免疫染色以Ki-67作為遠端大腸黏膜上皮細胞增生指標。結果顯示，在相同飼養條件下，不同鼠種的反應不盡相同：C57BL/6JNarl雄性小鼠各組平均體重差異大，而肝臟抗氧化酵素比活性組間差異較小；ICR雄性小鼠則是各組平均體重差異小，肝臟抗氧化酵素比活性組間差異較明顯。遠端大腸黏膜上皮細胞增生能力則在二鼠種間有相似之趨勢：93G組與M93G組有較低之增生能力。白米與米糠加糙米飼料使小鼠有較重體重，且攝食chow diet與米糠加糙米飼料，有助提升C57BL/6JNarl雄性小鼠肝臟抗氧化能力與ICR雄性小鼠肝臟及近端大腸抗氧化能力。因此，米食與非精製之飲食，具有提升組織抗氧化能力與疾病預防之潛力。

Rice has been the staple food and an important cash crop for Asians, including Taiwanese for a long time. There are abundant antioxidants and anti-inflammation substances in rice, most of them exist in the bran layer and the germ. According to the epidemiological studies, the more intake of those foods containing antioxidant substances, the less morbidity and mortality of human diseases. Therefore, replacing the polished rice with the whole grain rice diet should be a pro-health dietary pattern. Different from other studies using extracts from rice bran, this study used rice diet to investigate the effects on the antioxidant capacity of liver and colon tissue of mice. Forty-five male inbred C57BL/6JNarl mice were obtained from the National Laboratory Animal Center and forty-five male outbred ICR mice were obtained from the BioLasco Taiwan Co., Ltd. Mice were assigned to 5 dietary groups: an AIN-93G group (93G), a modified high protein AIN-93G group (M93G), a chow diet group (Chow), a polished rice high protein group (PR), and a bran-enriched brown rice high protein group (RB). The mice were fed with the experimental diets and the body weight and food intake were recorded routinely during the experiment. Mice were sacrificed after 4-week fed with experimental diets. Liver tissues were collected for analysis of the antioxidant related enzymes, such as glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT). Colon tissues were collected for analysis of glutathione (GSH) and the antioxidant enzymes, and immunochemistry assays of cell proliferation marker, Ki-67.
The results show the 2 mouse strains had different responses to the same rearing condition. The body weight of male C57BL/6JNarl mice had obvious difference, but most of the liver antioxidant enzymes had no significant differences. However, male ICR mice had converse results. The cell proliferation capacity in the mucosa epithelial cells of distal colon had a similar trend: the 93G group and M93G group had lower Ki-67 expression. The mice of both strains fed with the PR and RB diet were heavier than others. Chow diet and RB diet enhanced liver antioxidant enzymes specific activity of the 2 mouse strains, and so did in the proximal colon of male ICR mice. In conclusion, these results indicate rice diets and non-refined diets can increase tissue antioxidant capacity and have a great possibility to prevent disease.

中文摘要............................................................i
英文摘要............................................................ii
縮寫對照表.........................................................iii
第一章 文獻回顧....................................................1
第一節 研究動機與目的..............................................1
第二節 文獻回顧....................................................3
一、 自由基與活性氧化物質.........................................3
二、 氧化壓力...................................................6
三、 體內的抗氧化防禦系統.........................................7
（一） 抗氧化物....................................................7
（二） 抗氧化酵素..................................................12
四、 稻米與保健..................................................15
第二章 材料與方法..................................................19
一、 實驗設計與前言...............................................19
二、 實驗材料.....................................................21
（一） 飼料配製....................................................21
（二） 藥品與試劑..................................................21
（三） 儀器設備....................................................23
三、 實驗方法....................................................24
（一） 實驗動物....................................................24
（二） 動物分組與飼養流程..........................................24
（三） 實驗飼料....................................................25
（四） 動物犧牲....................................................32
（五） 組織固定與病理切片..........................................32
（六） 組織免疫染色................................................32
（七） 組織免疫染色定量............................................33
（八） 組織抗氧化分析..............................................33
（九） 統計分析....................................................37
第三章 結果.......................................................38
一、 C57BL/6JNarl小鼠..........................................38
（一） 小鼠體重變化、攝食量與飼料利用率.............................38
（二） 組織器官重量與大腸長度......................................38
（三） 組織免疫染色................................................39
（四） 肝臟抗氧化分析..............................................39
二、 ICR小鼠....................................................41
（一） 小鼠體重變化、攝食量與飼料利用率.............................41
（二） 組織器官重量與大腸長度......................................41
（三） 組織免疫染色...............................................42
（四） 組織抗氧化分析...........................................42
第四章　討論........................................................82
一、 攝食米食對小鼠體重變化之影響.................................82
二、 攝食米食對於小鼠組織抗氧化能力之影響.........................82
三、 攝食米食實驗飼料對於小鼠大腸之影響...........................84
四、 結論.........................................................85
第五章 未來展望....................................................88
第六章　參考文獻....................................................89
附錄一 動物實驗申請案送審收件證明................................98
附錄二 Lab diet 5001飼料組成.......................................99

中華民國國家標準（1994）。13446類號 N1126「稻米詞彙」。

吳映蓉（2006）。五色蔬果健康全書。台北市：臉譜。

徐鵬洋 （2009）。以小鼠化學致癌發炎模式探討米食對大腸直腸腫瘤發生之影響。國立台灣大學微生物與生化學研究所碩士論文。

陳惠英、顏國欽（1998）。自由基、抗氧化防禦與人體健康。中華民國營養學會雜誌 23：105-121。

賴明宏（2001）。飲食中補充米麩或三價鉻對第二型糖尿病患或STZ誘導的糖尿病大白鼠醣類及脂質代謝之影響。台北醫學院藥學研究所博士論文。

Abei, H. (1984). Catalase in vitro. Methods in Enzymology 105, 121-126.

Babior, B.M., and Curnutte, J.T. (1987). Chronic granulomatous disease--Pieces of a cellular and molecular puzzle. Blood Reviews 1, 215-218.

Barja, G. (2004). Free radicals and aging. Trends in Neurosciences 27, 595-600.

Board, P.G. (2007). The use of glutathione transferase-knockout mice as pharmacological and toxicological models. Expert Opinion on Drug Metabolism & Toxicology 3, 421-433.

Bosch, H.V.D., Schutgens, R.B.H., Wanders, R.J.A., and Tager, J.M. (1992). Biochemistry of Peroxisomes. Annual Review of Biochemistry 61, 157-197.

Burk, R.F. (1991). Molecular biology of selenium with implications for its metabolism. FASEB J 5, 2274-2279.

Carlsson, L.M., Jonsson, J., Edlund, T., and Marklund, S.L. (1995). Mice lacking extracellular superoxide dismutase are more sensitive to hyperoxia. Proc Natl Acad Sci U S A 92, 6264-6268.
Cavalieri, E.L., and Rogan, E.G. (1995). Central role of radical cations in metabolic activation of polycyclic aromatic hydrocarbons. Xenobiotica 25, 677-688.

Champagne, E.T., Horn, R.J., SR., and Abraham, G. (1991). Stailizing Brown Rice Products by Aqueous Ethanol Extraction. Cereal Chem 68, 267-271.

Cheng, W.-H., Gerald F. Combs, J., and Lei, X.G. (1998). Knockout of cellular glutathione peroxidase affects selenium-dependent parameters similarly in mice fed adequate and excessive dietary selenium. BioFactors 7, 311-321.

Cheng, W.-H., Ho, Y.-S., Ross, D.A., Valentine, B.A., Combs Jr., G.F., and Lei, X.G. (1997). Cellular Glutathione Peroxidase Knockout Mice Express Normal Levels of Selenium-Dependent Plasma and Phospholipid Hydroperoxide Glutathione Peroxidases in Various Tissues. J Nutr 127, 1445-1450.

Chopra, M., McLoone, M., O’Neill, N., and Williams, D. (1996). Fruit and vegetable supplementation- effect on ex-vivo LDL oxidation in humans. In Natural antioxidants and food quality in atherosclerosis and cancer prevention, J. Kumpulainen, and J. Salonen, eds. (Cambridge, UK, Royal Society of Chemistry), pp. 151-155.

Church, D.F., and Pryor, W.A. (1985). Free-radical chemistry of cigarette smoke and its toxicological implications. Environ Health Perspect 64, 111-126.

Devasagayam, T.P., Tilak, J.C., Boloor, K.K., Sane, K.S., Ghaskadbi, S.S., and Lele, R.D. (2004). Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India 52, 794-804.

Elchuri, S., Oberley, T.D., Qi, W., Eisenstein, R.S., Jackson Roberts, L., Van Remmen, H., Epstein, C.J., and Huang, T.T. (2005). CuZnSOD deficiency leads to persistent and widespread oxidative damage and hepatocarcinogenesis later in life. Oncogene 24, 367-380.

Erzurum, S.C., Lemarchand, P., Rosenfeld, M.A., Yoo, J.H., and Crystal, R.G. (1993). Protection of human endothelial cells from oxidant injury by adenovirus-mediated transfer of the human catalase cDNA. Nucleic Acids Res 21, 1607-1612.

Flohe, L., and Otting, F. (1984). Superoxide Dismutase Assays. Methods in Enzymology 105, 93-104.
Forman, H.J., Zhang, H., and Rinna, A. (2009). Glutathione: Overview of its protective roles, measurement, and biosynthesis. Molecular Aspects of Medicine 30, 1-12.

Graf, E., and Eaton, J.W. (1990). Antioxidant functions of phytic acid. Free Radic Biol Med 8, 61-69.

Green, M.J., and Hill, H.A.O. (1984). Chemistry of dioxygen. In Methods in Enzymology, P. Lester, ed. (Academic Press), pp. 3-22.

Halliwell, B. (1993). The role of oxygen radicals in human disease, with particular reference to the vascular system. Haemostasis 23 Suppl 1, 118-126.

Halliwell, B., and Gutteridge, J.M.C. (1999). Free radicals in biology and medicine (New York, Oxford University Press).

Halliwell, B., and Gutteridge, M.C. (1989). Free radicals, ageing and disease. In Free radicals in biology and medicine, B. Halliwell, and M.C. Gutteridge, eds. (Oxford, Clarendon Press), pp. 484-487.

Halliwell, B., Murcia, M.A., Chirico, S., and Aruoma, O.I. (1995). Free radicals and antioxidants in food and in vivo: what they do and how they work. Crit Rev Food Sci Nutr 35, 7-20.

Hashizume, K., Hirasawa, M., Imamura, Y., Noda, S., Shimizu, T., Shinoda, K., Kurihara, T., Noda, K., Ozawa, Y., Ishida, S., et al. (2008). Retinal Dysfunction and Progressive Retinal Cell Death in SOD1-Deficient Mice. The American Journal of Pathology 172, 1325-1331.

Hazlewood, C., and Davies, M.J. (1995). Damage to DNA and RNA by tumour promoter-derived alkoxyl radicals: an EPR spin trapping study. Biochem Soc Trans 23, 259S.

Hiramitsu, T., and Armstrong, D. (1991). Preventive effect of antioxidants on lipid peroxidation in the retina. Ophthalmic Res 23, 196-203.

Ho, Y.-S., Xiong, Y., Ma, W., Spector, A., and Ho, D.S. (2004). Mice Lacking Catalase Develop Normally but Show Differential Sensitivity to Oxidant Tissue Injury. THE JOURNAL OF BIOLOGICAL CHEMISTRY 279, 32804-32812.
Ho, Y.S., Gargano, M., Cao, J., Bronson, R.T., Heimler, I., and Hutz, R.J. (1998). Reduced fertility in female mice lacking copper-zinc superoxide dismutase. J Biol Chem 273, 7765-7769.

Huang, C.S., Chang, L.S., Anderson, M.E., and Meister, A. (1993). Catalytic and regulatory properties of the heavy subunit of rat kidney gamma-glutamylcysteine synthetase. J Biol Chem 268, 19675-19680.

Im, E., Choi, Y.J., Pothoulakis, C., and Rhee, S.H. (2009). Bacillus polyfermenticus ameliorates colonic inflammation by promoting cytoprotective effects in colitic mice. J Nutr 139, 1848-1854.

Imlay, J.A., and Fridovich, I. (1991). Assay of metabolic superoxide production in Escherichia coli. Journal of Biological Chemistry 266, 6957-6965.

Islam, M., Murata, T., Fujisawa, M., Nagasaka, R., Ushio, H., Bari, A., Hori, M., and Ozaki, H. (2008). Anti-inflammatory effects of phytosteryl ferulates in colitis induced by dextran sulphate sodium in mice. British Journal of Pharmacology 154, 812-824.

Jones, D.P. (2001). Bioavailability of Glutathione. In Handbook of Antioxidants, E. Cadenas, and L. Packer, eds. (Marcel Dekker).

Juliano, B.O. (1985). Rice : chemistry and technology (St. Paul, Minn., USA, American Association of Cereal Chemists).

Kalpakcioglu, B., and Şenel, K. (2008). The interrelation of glutathione reductase, catalase, glutathione peroxidase, superoxide dismutase, and glucose-6-phosphate in the pathogenesis of rheumatoid arthritis. Clinical Rheumatology 27, 141-145.

Kaneto, H., Kajimoto, Y., Miyagawa, J., Matsuoka, T., Fujitani, Y., Umayahara, Y., Hanafusa, T., Matsuzawa, Y., Yamasaki, Y., and Hori, M. (1999). Beneficial effects of antioxidants in diabetes: possible protection of pancreatic beta-cells against glucose toxicity. Diabetes 48, 2398-2406.

Kawabata, K., Tanaka, T., Murakami, T., Okada, T., Murai, H., Yamamoto, T., Hara, A., Shimizu, M., Yamada, Y., Matsunaga, K., et al. (1999). Dietary prevention of azoxymethane-induced colon carcinogenesis with rice-germ in F344 rats. Carcinogenesis 20, 2109-2115.
Kim, J.-S., Jung, J., Lee, H.-J., Kim, J.C., Wang, H., Kim, S.-H., Shin, T., and Moona, C. (2009). Differences in immunoreactivities of Ki-67 and doublecortin in the adult hippocampus in three strains of mice. Acta histochemica 111, 150-156.

Kim, J.Y., Do, M.H., and Lee, S.S. (2006). The Effects of a Mixture of Brown and Black Rice on Lipid Profiles and Antioxidant Status in Rats. Annals of Nutrition & Metabolism 50, 347-353.

Kim, S.J., Han, D., Moon, K.D., and Rhee, J.S. (1995). Measurement of superoxide dismutase-like activity of natural antioxidants. Biosci Biotechnol Biochem 59, 822-826.

Kim, S.M., Rico, C.W., Lee, S.C., and Kang, M.Y. (2010). Modulatory Effect of Rice Bran and Phytic Acid on Glucose Metabolism in High Fat-Fed C57BL/6N Mice. J Clin Biochem Nutr 47, 12-17.

Lai, P., Li, K.Y., Lu, S., and Chen, H.H. (2009). Phytochemicals and antioxidant properties of solvent extracts from Japonica rice bran. Food Chemistry 117, 538-544.

Lebovitz, R.M., Zhang, H., Vogel, H., Cartwright, J., Jr., Dionne, L., Lu, N., Huang, S., and Matzuk, M.M. (1996). Neurodegeneration, myocardial injury, and perinatal death in mitochondrial superoxide dismutase-deficient mice. Proc Natl Acad Sci U S A 93, 9782-9787.

Li, Y., Huang, T.T., Carlson, E.J., Melov, S., Ursell, P.C., Olson, J.L., Noble, L.J., Yoshimura, M.P., Berger, C., Chan, P.H., et al. (1995). Dilated cardiomyopathy and neonatal lethality in mutant mice lacking manganese superoxide dismutase. Nat Genet 11, 376-381.

Liao, J., Seril, D.N., Yang, A.L., Lu, G.G., and Yang, G.-Y. (2006). Inhibition of chronic ulcerative colitis associated adenocarcinoma development in mice by inositol compounds. Carcinogenesis 28, 446-454.

Lindau-Shepard, B.A., and Shaffer, J.B. (1993). Expression of human catalase in acatalasemic murine SV-B2 cells confers protection from oxidative damage. Free Radic Biol Med 15, 581-588.

Lipinski, B. (2001). Pathophysiology of oxidative stress in diabetes mellitus. J Diabetes Complications 15, 203-210.
Lopez-Barea, J., Barcena, J.A., Bocanegra, J.A., Florindo, J., Garcia-Alfonso, C.L.-R., A., Martinez-Galisteo, E., and Peinado, J. (1990). Structure, mechanism, functions and regulatory properties of glutathione reductase. In Glutathione : metabolism and physiological functions, J. Vina, ed. (Boca Raton, CRC Press), pp. 105-116.

Maher, P. (2005). The effects of stress and aging on glutathione metabolism. Ageing Research Reviews 4, 288-314.

MAIER, C.M., and CHAN, P.H. (2002). Role of Superoxide Dismutases in Oxidative Damage and Neurodegenerative Disorders. The Neuroscientist 8, 323-334.

MALY, F.-E. (1990). THE B LYMPHOCYTE: A NEWLY RECOGNIZED SOURCE OF REACTIVE OXYGEN SPECIES WITH IMMUNOREGULATORY POTENTIAL. Free Rud Res Contms 8, 143-148.

Meier, B., Radeke, H.H., Selle, S., Raspe, H.H., Sies, H., Resch, K., and Habermehl, G.G. (1990). Human fibroblasts release reactive oxygen species in response to treatment with synovial fluids from patients suffering from arthritis. Free Radic Res Commun 8, 149-160.

Minamiyama, Y., Yoshikawa, T., Tanigawa, T., Takahashi, S., Naito, Y., Ichikawa, H., and Kondo, M. (1994). Antioxidative effects of a processed grain food. J Nutr Sci Vitaminol (Tokyo) 40, 467-477.

Modak, M., Dixit, P., Londhe, J., Ghaskadbi, S., and Devasagayam, T.P.A. (2007). Indian Herbs and Herbal Drugs Used for the Treatment of Diabetes. Journal of Clinical Biochemistry and Nutrition 40, 163-173.

Murakami, K., Inagaki, J., Saito, M., Ikeda, Y., Noda, C.T., Kawakami, S., Shirasawa, T., and Shimizu, T. (2009). Skin atrophy in cytoplasmic SOD-deficient mice and its complete recovery using a vitamin C derivative. Biochemical and Biophysical Research Communications 382, 457-461.

Norazalina, S., Norhaizan, M.E., Hairuszah, I., and Norashareena, M.S. (2010). Anticarcinogenic efficacy of phytic acid extracted from rice bran on azoxymethane-induced colon carcinogenesis in rats. Experimental and Toxicologic Pathology 62, 259-268.

Oyama, T., Yasui, Y., Sugie, S., Koketsu, M., Watanabe, K., and Tanaka, T. (2009). Dietary Tricin Suppresses Inflammation-Related Colon Carcinogenesis in Male Crj: CD-1 Mice. Cancer Prev Res 2, 1031-1038.

Poyer, J.L., McCay, P.B., Lai, E.K., Janzen, E.G., and Davis, E.R. (1980). Confirmation of assignment of the trichloromethyl radical spin adduct detected by spin trapping during 13C-carbon tetrachloride metabolism in vitro and in vivo. Biochem Biophys Res Commun 94, 1154-1160.

Pryor, W.A., Lightsey, J.W., and Church, D.F. (1982). Reaction of nitrogen dioxide with alkenes and polyunsaturated fatty acids: addition and hydrogen-abstraction mechanisms. Journal of the American Chemical Society 104, 6685-6692.

Qiao, M., Kisgati, M., Cholewa, J.M., Zhu, W., Smart, E.J., Sulistio, M.S., and Asmis, R. (2007). Increased Expression of Glutathione Reductase in Macrophages Decreases Atherosclerotic Lesion Formation in Low-Density Lipoprotein Receptor-Deficient Mice. Arterioscler Thromb Vasc Biol 27, 1375-1382.

Qureshi, A.A., Salser, W.A., Parmar, R., and Emeson, E.E. (2001). Novel Tocotrienols of Rice Bran Inhibit Atherosclerotic Lesions in C57BL/6 ApoE-Deficient Mice. J Nutr 131, 2606-2618.

Qureshi, A.A., Sami, S.A., and Khan, F.A. (2002). Effects of stabilized rice bran, its soluble and fiber fractions on blood glucose levels and serum lipid parameters in humans with diabetes mellitus Types I and II. The Journal of Nutritional Biochemistry 13, 175-187.

Reid, T.M., and Loeb, L.A. (1993). Effect of DNA-repair enzymes on mutagenesis by oxygen free radicals. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 289, 181-186.

Saul, R.L., and Ames, B.N. (1986). Background levels of DNA damage in the population. Basic Life Sci 38, 529-535.

Sentman, M.-L., Granström, M., Jakobson, H., Reaume, A., Basu, S., and Marklund, S.L. (2006). Phenotypes of Mice Lacking Extracellular Superoxide Dismutase and Copper- and Zinc-containing Superoxide Dismutase. Journal of Biological Chemistry 281, 6904-6909.
Shamsuddin, A.M., Vucenik, I., and Cole, K.E. (1997). IP6: A novel anti-cancer agent. Life Sciences 61, 343-354.

Sharma, R.A., Manson, M.M., Gescher, A., and Steward, W.P. (2001). Colorectal cancer chemoprevention: biochemical targets and clinical development of promising agents. Eur J Cancer 37, 12-22.

Sies, H. (1996). Antioxidants in disease mechanisms and therapy (New York, Plenum Publishers).

Simic, M.G. (1988). Mechanisms of inhibition of free-radical processes in mutagenesis and carcinogenesis. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 202, 377-386.

Sims, N.R., Nilsson, M., and Muyderman, H. (2004). Mitochondrial glutathione: a modulator of brain cell death. J Bioenerg Biomembr 36, 329-333.

Sowbhagya, C.M., and Bhattacharya, K.R. (1976). LIPID AUTOXIDATION IN RICE. Journal of Food Science 41, 1018-1023.

Stadtman, E.R., and Oliver, C.N. (1991). Metal-catalyzed oxidation of proteins. Physiological consequences. J Biol Chem 266, 2005-2008.

Surh, Y.J. (2003). Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer 3, 768-780.

Suzuki, R., Kohno, H., Sugie, S., and Tanaka, T. (2004). Sequential observations on the occurrence of preneoplastic and neoplastic lesions in mouse colon treated with azoxymethane and dextran sodium sulfate. Cancer Science 95, 721-727.

Tanaka, T., Kohno, H., Suzuki, R., Yamada, Y., Sugie, S., and Mori, H. (2003). A novel inflammation-related mouse colon carcinogenesis model induced by azoxymethane and dextran sodium sulfate. Cancer Science 94, 965-973.

Verschoyle, R.D., Greaves, P., Cai, H., Edwards, R.E., Steward, W.P., and Gescher, A.J. (2007). Evaluation of the cancer chemopreventive efficacy of rice bran in genetic mouse models of breast, prostate and intestinal carcinogenesis. Br J Cancer 96, 248-254.

Wang, M.Y., and Liehr, J.G. (1995). Lipid Hydroperoxide-Induced Endogenous DNA Adducts in Hamsters: Possible Mechanism of Lipid Hydroperoxide-Mediated Carcinogenesis. Archives of Biochemistry and Biophysics 316, 38-46.

Winterbourn, C.C., and Stern, A. (1987). Human red cells scavenge extracellular hydrogen peroxide and inhibit formation of hypochlorous acid and hydroxyl radical. J Clin Invest 80, 1486-1491.

Wozniak, D.F., Brosnan-Watters, G., Nardi, A., McEwen, M., Corso, T.D., Olney, J.W., and Fix, A.S. (1996). MK-801 neurotoxicity in male mice: histologic effects and chronic impairment in spatial learning. Brain Research 707, 165-179.

Yoo, J.H., Erzurum, S.C., Hay, J.G., Lemarchand, P., and Crystal, R.G. (1994). Vulnerability of the human airway epithelium to hyperoxia. Constitutive expression of the catalase gene in human bronchial epithelial cells despite oxidant stress. The Journal of Clinical Investigation 93, 297-302.

Zhao, Q., Murakami, Y., Tohda, M., Obi, R., Shimada, Y., and Matsumoto, K. (2007). Chotosan, a Kampo Formula, Ameliorates Chronic Cerebral Hypoperfusion-Induced Deficits in Object Recognition Behaviors and Central Cholinergic Systems in Mice. J Pharmacol Sci 103, 360-373.