臺灣博碩士論文加值系統

本研究建立馬來鹿角蕨(Platycerium ridleyi Christ.)以綠球體繁殖之組織培養流程，取幼葉培殖體培養在添加5.37 μM α-naphthaleneacetic acid (NAA)之MS培養基中，可誘導出綠球體(green globular body; GGB)。上葉(葉前端)及下葉(葉基部)培殖體誘導綠球體的效果並無顯著差異。在綠球體組織均質化接種試驗中，每培養皿以0.05 g鮮重組織接種後，幼孢子體有最佳再生效果，經取樣估算每皿可獲得121.2個幼孢子體，優於接種0.1、0.15及0.2 g者(p < 0.05)。吸附劑試驗顯示，添加活性碳(activated charcoal; AC)有助於幼孢子體的再生，接種量0.05 g並於培養基中添加2.5 g L-1 AC有最多的幼孢子體數量達362.0/皿，若添加聚乙烯吡咯烷酮(polyvinylpyrrolidone; PVP)及交聯聚乙烯吡咯烷酮(polyvinylpolypyrrolidone; PVPP)，則效果沒有AC佳。待幼孢子體長度達1-1.5 cm時再利用生長素(auxin)進行發根誘導，以6 μM indolebutyric acid (IBA)有最佳發根率(80.0%)及發根數量(4.0/explant)。在健化過程中以有根之孢子體移植在泥炭土介質中，存活率可達100%及根系數量達28.8/ explant。

The present study developed an in vitro propagation system for Platycerium ridleyi Christ. via induction of green globular body (GGB). The induction was achieved by using juvenile leaves as explants incubating on Murashige and Skoog (MS) medium supplemented with 5.37μM α-naphthalene acetic acid (NAA). There was no significant difference in the GGB induction using either proximal or distal half leaves as explants. The inoculation test of homogenized GGB tissues revealed that the single inoculum with 0.05 g fresh tissue per Petri dish was better than those of 0.1, 0.15 and 0.2 g (p < 0.05); and subsequently obtained a production of 121.2 shoots per dish. The addition of 2.5 g L-1 activated charcoal (AC) on culture medium with 0.05 g dish-1 inoculum greatly enhanced the regeneration of juvenile sporophytes (362.0/dish). However, the addition of polyvinylpyrrolidone (PVP) or polyvinylpolypyrrolidone (PVPP) was less effect as compared to AC. The elongated sporophytes (1-1.5 cm in length) were rooted successfully by auxin stimulation where a 6 μM-indolebutyric acid (IBA) treatment achieved the best rooting percentage (80%) and also obtained the greatest root number (4.0/explant). Rooted shoots performed 100% survival rate and maintained greater root number (28.8/explant) through the acclimatization trials when planted in the peat moss rooting medium.

Ⅰ、前言 1
Ⅱ、前人研究 3
(Ⅰ)鹿角蕨屬植物介紹 3
(Ⅱ)鹿角蕨的有性繁殖 5
(Ⅲ)鹿角蕨的無性繁殖 5
1.分株繁殖 5
2.組織培養 6
(Ⅳ)抗褐化劑 11
(Ⅴ)植株發根及健化 13
Ⅲ、材料與方法 15
(Ⅰ)植物材料 15
(Ⅱ)綠球體誘導 15
(Ⅲ)綠球體增殖試驗 15
(Ⅳ)綠球體形態之石蠟切片觀察 16
(Ⅴ)孢子體再生 17
1.綠球體組織均質化接種試驗 17
2.吸附劑添加試驗 19
(Ⅵ)誘導發根 20
(Ⅶ)試管外發根、健化介質之選擇 20
(Ⅷ)培養條件 21
(Ⅸ)資料處理與分析 22
Ⅳ、結果 24
(Ⅰ)不同部位葉培殖體對綠球體誘導影響 24
(Ⅱ)綠球體之增殖 24
(Ⅲ)綠球體形態之石蠟切片觀察 28
(Ⅳ)孢子體再生 30
1.決定有效再生芽體的顯微觀察 30
2.均質綠球體接種量試驗 32
3.AC吸附試驗 35
4.PVP吸附試驗 38
5.PVPP吸附試驗 41
(Ⅴ)誘導發根 44
(Ⅵ)試管外發根介質之選擇 46
Ⅴ、討論 49
Ⅵ、參考文獻 58
Ⅶ、附錄 68

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