臺灣博碩士論文加值系統

本論文研究運用乙醯膽鹼酯酶檢測法，並使用樹莓派(Raspberry Pi 3B+)、網路攝影機Logitech C310、QT Creator及運用3D設計和3D列印技術。
研究中使用了乙醯膽鹼酯酶(Acetylcholinesterase)在農藥檢測時化學反應之RGB 三原色變化進行色彩辨識與影像擷取，再存取RGB數據及積分面積，使OriginPro8 的Fitting 功能產生之公式計算出積分面積所對應的 PPM，而將公式放入程式中，當我們放入含有農藥檢測液進路針筒中，而將基質放入玻璃試瓶中，點選人機介面上的開始按鈕就會觸發電磁閥開啟，含有農藥的檢測液會經由電磁閥流入玻璃試瓶中，攝影機擷取影像辨識，經過測試秒數20秒後程式就會以套入的公式進行PPM計算而計算結束後就會呈現再出積分面積所對應的PPM，我們也能使用手機取得樹莓派檢測後的數據(R、G、B、PPM、Time)，而在我們按下人接介面的開始鈕時數據會以陣列的方式存取及以時間來作為檔名，這樣存檔就不會因數據會重疊也方便我們日後能夠研究。
本論文也藉由 TCP 的客戶端與伺服器模式在不需要網路的時候也能離線傳送資料，樹莓派成為伺服器手機當作為客戶端，傳送資料前須將樹莓派(Raspberry Pi 3B+)設定為 AP模式作為Wifi分享器，手機端與樹莓派須加入QTCPsocket、QTcpServer，樹莓派中的listen函數開始接受手機(Client)連線就會出發傳送資料的訊號槽，傳送資料的訊號槽就會傳送資料讓手機讀取，在資料傳送時使用QString date2 =QString::number將整數或浮點數轉成字串來傳送，而本研究加入的這項功能方便又清晰的從手機看到檢測數據。

In this paper, acetylcholinesterase detection method was used, Raspberry Pi 3B+, Logitech C310, QT Creator and 3D design and printing technology were used.
In this study, Acetylcholinesterase was used for color recognition and image capture of the RGB primary color changes of chemical reactions during pesticide detection, and RGB data and integral area were accessed to calculate the PPM corresponding to the integral area by the formula of Fitting function of Origin Pro8. The formula was put into the program. When we put the matrix into the glass bottle, the start button on the interface will trigger the solenoid valve to open. The detection liquid containing the pesticide will flow into the glass bottle through the solenoid valve. The camera picks up the image and identifies it. After 20 seconds of testing, the program will be inserted. The formula of PPM calculates and shows the PPM corresponding to the integral area after calculating. We can also use mobile phones to obtain the data after raspberry pie detection (R, G, B, PPM, Time), and when we press the start button of the human interface, the data will be accessed in an array way and the file name will be time, so that the file will be saved. It will not overlap the data and will be convenient for us to study in the future.
This paper also uses TCP client and server mode to transmit data offline without network. Raspberry pie becomes a server mobile phone as a client. Raspberry Pi 3B+ should be set as AP mode as Wifi Sharer before data transmission. Both mobile phone and raspberry pie need to add QTCPsocket and QTcpServer. The listen function in the raspberry pie begins to accept the connection of the mobile phone (Client) and starts to send the data into the slot. The slot transmits the data for the mobile phone to read. QString date 2 = QString:: Number is used to transfer the integer or floating-point number into a string for transmission. This function is convenient to add in this study. And clearly from the mobile phone to see the detection data.

摘要.........i
Abstract.........ii
誌謝.........iii
目錄.........iv
表目錄.........vi
圖目錄.........vii
第一章 緒論.........1
1.1 Raspberry Pi簡介.........1
1.2乙醯膽鹼酯酶-農藥殘留檢測法介紹.........2
1.3研究動機.........3
第二章 文獻探討.........4
2.1 Raspberry Pi 硬體與歷史介紹.........4
2.2乙醯膽鹼中毒反應.........5
2.3 OpenCV與光的三原色介紹.........6
第三章 環境建置與實驗方法.........7
3.1實驗軟硬體架構與檢測軟體設計流程圖.........7
3.1.1實驗架構軟硬體流程圖.........7
3.1.2檢測軟體設計流程圖.........7
3.2 Raspberry Pi Raspbian系統安裝.........8
3.2.1 Raspbian ISO軟體下載.........8
3.2.2 Win32 Disk Imager ISO映像檔燒錄軟體安裝.........9
3.2.3 Raspbian作業系統安裝.........9
3.3 Raspberry Pi 7吋觸控螢幕安裝.........10
3.3.1觸控螢幕線材連接.........10
3.3.2觸控螢幕開機測試.........10
3.4 VNC遠端控制系統安裝.........11
3.4.1開啟Raspberry Pi VNC功能.........11
3.4.2個人電腦安裝VNC Viewer遠端控制軟體.........12
3.4.3 Raspberry Pi浮動IP查詢.........12
3.4.4 VNC Viewer連線與測試.........13
3.5 QT Creator人機介面開發軟體安裝.........14
3.5.1安裝QT Creator.........15
3.5.2 QT Creator編譯器環境參數設定.........15
3.6安裝樹莓派3B+作為WIFI 分享器.........18
3.7手機APP與樹莓派傳輸資料.........20
3.8 檢測儀器架構3D設計與組裝.........25
3.8.1 安裝Autodesk 123D Design繪圖軟體.........25
3.8.2 Autodesk 123D Design設計與輸出.........25
3.8.3 Autodesk 123D Design列印.........27
3.8.4 硬體架構組裝.........29
3.9 攝影機參數調整.........31
3.10 影像處理程式說明.........32
3.11實驗流程與原理.........37
3.11.1乙醯膽鹼酯酶檢測原理.........37
3.11.2實驗檢測-自備標準濃度農藥殘留液.........38
3.12.3農藥檢測液配製.........40
第四章 結果與討論.........45
4.1各濃度RGB數值量測數據.........45
4.2緩衝液4毫升之農藥濃度量測.........53
4.3以純水稀釋農藥進行濃度檢測.........61
第五章 結論.........69
參考文獻.........70
Extended Abstract.........72

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