臺灣博碩士論文加值系統

乳癌為全球女性居高不下的致死及致病率。因此，必須提高治療乳癌的效率和開發出更便利之治療乳癌的新方法。光熱治療為一種新型治療癌症的方式，利用近紅外光的熱產生局部高溫，熱殺腫瘤細胞，與傳統癌症療法相比具有微創、副作用低、準確治療等優點，在癌症治療領域具有極佳之發展潛力。
本研究利用一次性合成，使用聚多巴胺 ( Polydopamine ) 還原氧化石墨烯和將零價鐵 ( zero valent iron；ZVI ) 直接結合上還原氧化石墨烯，形成複合式材料ZVI-pDA/rGO，搭配紅外光之照射將同時兼具光動和光熱。研究中將評估ZVI-pDA/rGO奈米藥物之生物相容性、對乳癌細胞MCF-7之殺傷效率。實驗結果顯示，由於ZVI-pDA/rGO具備低生物毒性、較高的近紅外光吸光效率、光熱轉換為24.08 ％、光動治療效率，因此可以殺死56 ％乳癌細胞。此外，ZVI附著於pDA/rGO表面確實可以提高活性氧物質( Reactive oxygen species；ROS )的表現。本研究證實ZVI-pDA/rGO結合了ZVI、多巴胺與rGO的特點，具備極佳的生物相容性及抗乳癌細胞功能，顯示ZVI-pDA/rGO對乳癌之治療具極高的發展潛力。

Breast cancer is a cause of death and morbidity for women throughout the world. Therefore, it is necessary to improve the efficiency of treating breast cancer and develop a new and easy method for treating breast cancer. Phototherapy utilizes near-infrared light to generate local heat and ROS that kill tumor cells. Compared with the traditional cancer therapy, it has the advantages of minimal invasion, low side effects, accurate treatment, and has the excellent potential for the development in the field of cancer treatment.
In this study, polydopamine was used to reduce graphene oxide and zero-valent iron (ZVI) directly bonded to the reduced graphene oxide that formed composite material ZVI-pDA/rGO at the same time. The infrared light irradiation on ZVI-pDA/rGO will also have the functions of photodynamic, thermo-optical. The study will evaluate the biocompatibility of ZVI-pDA/rGO nanomedicine, the killing efficiency of breast cancer cell MCF-7. The experimental results show that ZVI-pDA/rGO with low biological toxicity, high near-infrared light absorption photothermal conversion, photodynamic treatment efficiency. Can efficiently kill breast cancer cells. This study confirms that ZVI-pDA/rGO combines the characteristics of ZVI, dopamine and rGO, and has excellent biocompatibility, the function of killing breast cancer cell. ZVI-pDA/rGO has showed the extremely high development potential in treatment of breast cancer.

摘要.......................................................................i
Abstract................................................................ii
誌謝....................................................................iii
目錄....................................................................iv
表目錄....................................................................vi
圖目錄.................................................................vii
縮寫對照表...............................................................viii
第一章 緒論.................................................................1
1.1前言....................................................................1
1.2研究動機及目的...........................................................2
第二章 文獻探討.............................................................3
2.1乳癌介紹.................................................................3
2.2光熱療法應用.............................................................4
2.2.1氧化石墨稀 ( Graphene oxide ; GO ).....................................5
2.2.2 聚多巴胺還原GO ( Polydopamine reduction of GO ; pDA-rGO ).............6
2.3 光動療法( Photodynamic therapy，PDT )...................................7
2.3.1 奈米零價鐵（Nano-zero-Valent iron ; nFe0）............................8
2.4 MRI影像的光熱治療奈米材料應用 ...........................................9
第三章 材料方法 ..........................................................11
3.1材料 ..................................................................11
3.2方法 ..................................................................12
3.2.1製備GO、pDA/rGO、ZVI .................................................12
3.2.2 合成ZVI-pDA/rGO.....................................................13
3.2.3 製備TEM、AFM、FTIR 樣品..............................................13
3.2.4 升溫曲線.............................................................13
3.2.5 光熱轉換效率.........................................................14
3.2.7 光熱治療.............................................................14
3.2.8 光動治療.............................................................15
3.2.9 材料靶向性 ..........................................................15
3.2.10 普魯士藍 ..........................................................15
第四章 結果與討論..........................................................16
4.1 GO，pDA / rGO，ZVI-pDA/rGO之物化分析...................................16
4.2 GO，pDA / rGO，ZVI-pDA/rGO氧化及還原之特性..............................20
4.3 GO，pDA / rGO和ZVI-pDA/rGO之光熱特性 ..................................22
4.4 GO，pDA / rGO和ZVI-pDA/rGO在MCF-07中的細胞毒活性及光熱治療...............25
4.5 GO，pDA / rGO和ZVI-pDA/rGO氧化壓力之測定...............................27
4.6 GO，pDA / rGO和ZVI-pDA/rGO之靶向性測定.................................29
4.7 測定ZVI-pDA/rGO零價鐵之氧化情況........................................30
第五章 結論...............................................................32
參考文獻 .................................................................33
Extended Abstract........................................................38

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